Team:Paris/September 2

From 2008.igem.org

(Difference between revisions)
(→Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3))
(→Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3))
Line 1: Line 1:
{{Paris/Calendar_Links|September 1|September 3}}
{{Paris/Calendar_Links|September 1|September 3}}
-
=Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3)=
+
=Ligation L173 ('''rbs-TetR''' in '''mRFP-LVA-tripart'''-pSB1A3)=

Revision as of 16:24, 2 September 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3)

Name Digestion number Digested by
rbs-TetR D112 EcoRI & SpeI (insert)
mRFP-LVA-tripart D187 EcoRI & XbaI (vector)


Control Sample
3:1 (insert:vector) ratio 4:1 (insert:vector) ratio
D112 (insert) 0 µL 1,4 µL 1,9 µL
D187 (vector) 2,5 µL 2,5 µL 2,5 µL
T4 DNA ligase 10X buffer 2 µL 2 µL 2 µL
T4 DNA ligase 1 µL 1 µL 1 µL
water 14,5 µL 13,1 µL 12,6 µL
Retrieved from "http://2008.igem.org/Team:Paris/September_2"