From 2008.igem.org
(Difference between revisions)
|
|
| Line 56: |
Line 56: |
| | |- | | |- |
| | |'''Expected size''' | | |'''Expected size''' |
| - | |
| |
| - | |
| |
| - | |
| |
| - | |
| |
| | |colspan="2"|1894 bp | | |colspan="2"|1894 bp |
| - | |
| |
| | |- | | |- |
| | |'''Measured size''' | | |'''Measured size''' |
Revision as of 15:58, 4 September 2008
|
← Yesterday ↓ Calendar ↑Tomorrow →
|
← Yesterday ↓ Calendar ↑Tomorrow →
PCR Screening of L173 transformants
Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3)
Ligation L173
- insert: D112 (ES) rbs-TetR
- vector: D187 (EX) mRFP-LVA-tripart-pSB1A3
| Sample
| positive control
pUC19
| negative control
no DNA
| ligation control
(without insert)
| L173 (3:1 ratio)
| L173 (4:1 ratio)
|
| Number of colonies
| many
| 1
| 0
| 2
| 0
|
PCR Screening
PCR screening programm
- elongation tim: 2 min
- primers used: O18 & O19
- positive PCR control: S158 (pSB3K3)
- negative PCR control: no template
| Well n°
| 1
| 2
| 3
| 4
| 5
| 6
| 7
|
| Sample
| 1 kb DNA ladder
| positive PCR control
| negative PCR control
| negative transformation control clone
| L173.1
| L173.2
| 100 bp DNA ladder
|
| Expected size
| 1894 bp
|
| Measured size
|
|
|
Transformation results
L177 = d134 + d187 (pFlhB-mRFP LVA+)
L175 = d149 + d° (pFliL - ECFP LVA+)
L176 = d149 + d° (pFliL - ECFP LVA-)
|
|
"
