Team:Paris/September 5

From 2008.igem.org

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(→Purification of D112 (rbs-TetR))
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{{Paris/Calendar_Links|September 4|September 6}}
{{Paris/Calendar_Links|September 4|September 6}}
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=Purification of D112 (rbs-TetR)=
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=Purification of yesterday digestion=
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{|border="1" style="text-align: center"
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|'''Digestion n°'''
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|'''DNA substrat'''
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|'''Digestion by'''
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|-
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|D112
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|MP106: S03879 (rbs-TetR) in pSB1A2
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|EcoRI & SpeI
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|-
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|}
==Electrophoresis and gel extraction==
==Electrophoresis and gel extraction==

Revision as of 16:08, 5 September 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Purification of yesterday digestion

Digestion n° DNA substrat Digestion by
D112 MP106: S03879 (rbs-TetR) in pSB1A2 EcoRI & SpeI

Electrophoresis and gel extraction

D112 before gel excision
after gel excision

1% agarose gel

Expected size Measured size
pSB1A2 (vector) 2079 bp 2 kb
rbs-TetR (insert) 703 bp 0,7 kb

Qiaquick Gel Extraction Kit

  • elution in 30 µL of EB buffer

Concentration measurement

Sample DNA concentration A260/A280
D112 8 µg/mL 2,31
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