Team:Paris/September 8

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(Difference between revisions)
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         * then we will try to ligate it back using our ligases.
         * then we will try to ligate it back using our ligases.
==condition used ==
==condition used ==
 +
{|border="1" style="text-align: center"
 +
|'''ligase'''
 +
|'''incubation time'''
 +
|'''amount of enzyme'''
 +
|-
 +
|L1 (ligase 1)
 +
|2h00
 +
|2 U (unite)
 +
|-
 +
|L1
 +
|2h00
 +
|400 U (unite)
 +
|-
 +
|L1
 +
|O/N
 +
|2U
 +
|-
 +
|L1
 +
|O/N
 +
|400U
 +
|-
 +
|L2 (ligase 2)
 +
|2h00
 +
|2 U (unite)
 +
|-
 +
|L2
 +
|2h00
 +
|400 U (unite)
 +
|-
 +
|L2
 +
|O/N
 +
|2U
 +
|-
 +
|L2
 +
|O/N
 +
|400U
 +
|}

Revision as of 14:06, 9 September 2008

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Contents

Checking our ligases

Since our ligation experiments didn't work during these few days, we presume that our problem came from the ligases. That's why we decided to check the activity of the ligases in our possession. To do so:* we will digest a plasmid with only one restriction enzyme (EcoRI). 

        * then we will try to ligate it back using our ligases.

condition used

ligase incubation time amount of enzyme
L1 (ligase 1) 2h00 2 U (unite)
L1 2h00 400 U (unite)
L1 O/N 2U
L1 O/N 400U
L2 (ligase 2) 2h00 2 U (unite)
L2 2h00 400 U (unite)
L2 O/N 2U
L2 O/N 400U



==

njnoinjo
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