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Team:Paris/August 28
From 2008.igem.org
(Difference between revisions)
(→Construction of pFlhB - mRFP Tripart (LVA+)) |
(→Cloning of EnvZ*) |
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==Digestion== | ==Digestion== | ||
| - | [[Image:EnvZ pSB1A2.JPG|thumb|Digestion of EnvZ* and lasR- | + | [[Image:EnvZ pSB1A2.JPG|thumb|Digestion of EnvZ* and lasR-pSB1A2]] |
{|border="1" style="text-align: center" | {|border="1" style="text-align: center" | ||
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*EnvZ*: directly by the Qiagen kit | *EnvZ*: directly by the Qiagen kit | ||
*pSB1A2: excision of the band of interest (2 kb) and then purification using the Qiagen kit | *pSB1A2: excision of the band of interest (2 kb) and then purification using the Qiagen kit | ||
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='''Digestion'''= | ='''Digestion'''= | ||
Revision as of 19:07, 9 September 2008
Transformation of OmpR*Cloning of EnvZ*The cloning of EnvZ* didn't worked. So we decided to try one more time, starting from the PCR product. Digestion
Digestion |
