Team:Paris/September 12

From 2008.igem.org

(Difference between revisions)
(Checking our ligases (following))
(Checking our ligases (following))
Line 33: Line 33:
|D202 <br> 2 µL
|D202 <br> 2 µL
|D202 <br> 3 µL
|D202 <br> 3 µL
-
|Ligation 1
+
|1
-
|Ligation 2
+
|2
-
|Ligation 3
+
|3
-
|Ligation 4
+
|4
-
|Ligation 5
+
|5
-
|Ligation 6
+
|6
-
|Ligation 7
+
|7
|100 bp ladder
|100 bp ladder
|}
|}

Revision as of 13:51, 12 September 2008

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Checking our ligases (following)

Analysis of the ligation products by electrophosesis

Ligation of the plasmid digested by EcoRI
Ligation of the plasmid digested by BspHI

1% agarose gel

Analysis of the ligation of the plasmid digested by EcoRI

well n° 1 2 3 4 5 6 7 8 9 10 11 12 13
sample 1 kb ladder MP101
0,5 µL
MP101
1 µL
D202
2 µL
D202
3 µL
1 2 3 4 5 6 7 100 bp ladder