Team:Paris/September 12

From 2008.igem.org

(Difference between revisions)
(→Checking our ligases (following))
(→Checking our ligases (following))
Line 11: Line 11:
'''Analysis of the ligation of the plasmid digested by EcoRI'''
'''Analysis of the ligation of the plasmid digested by EcoRI'''
 +
No heat inactivation of the ligase
{|border="1" style="text-align: center"
{|border="1" style="text-align: center"
|'''well n°'''
|'''well n°'''
Line 28: Line 29:
|-
|-
|'''sample'''
|'''sample'''
-
|1 kb ladder
+
|rowspan="3"|1 kb ladder
-
|MP101 <br> 0,5 µL
+
|rowspan="3|MP101
-
|MP101 <br> 1 µL
+
|rowspan="3|MP101  
-
|D202 <br> 2 µL
+
|rowspan="3"|D202  
-
|D202 <br> 3 µL
+
|rowspan="3"|D202
|1
|1
|2
|2
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|6
|6
|7
|7
-
|100 bp ladder
+
|rowspan="3"|100 bp ladder
 +
|-
 +
|'''purification <br> digestion products'''
 +
|colspan="3"|gel extraction
 +
|colspan="3"|column
 +
|no <br> purification
 +
|-
 +
|'''ligase used'''
 +
|L1
 +
|L2
 +
|L3
 +
|L1
 +
|L2
 +
|L3
 +
|L1
 +
|-
 +
|'''amount loaded'''
 +
|10 µL
 +
|0,5 µL
 +
|1 µL
 +
|2 µL
 +
|3 µL
 +
|colspan="6"|10 µL
 +
|3 µL
 +
|10 µL
|}
|}

Revision as of 13:59, 12 September 2008

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Checking our ligases (following)

Analysis of the ligation products by electrophosesis

Ligation of the plasmid digested by EcoRI
Ligation of the plasmid digested by BspHI

1% agarose gel

Analysis of the ligation of the plasmid digested by EcoRI No heat inactivation of the ligase

well n° 1 2 3 4 5 6 7 8 9 10 11 12 13
sample 1 kb ladder MP101 MP101 D202 D202 1 2 3 4 5 6 7 100 bp ladder
purification
digestion products 		gel extraction column no
purification
ligase used L1 L2 L3 L1 L2 L3 L1
amount loaded 10 µL 0,5 µL 1 µL 2 µL 3 µL 10 µL 3 µL 10 µL
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