Revision as of 14:02, 12 September 2008

Checking our ligases (following)

Ligation of the plasmid digested by EcoRI

Ligation of the plasmid digested by BspHI

1% agarose gel

Analysis of the ligation of the plasmid digested by EcoRI No heat inactivation of the ligase

well n°	1	2	3	4	5	6	7	8	9	10	11	12	13
sample	1 kb ladder	MP101	MP101	D202	D202	1	2	3	4	5	6	7	100 bp ladder
purification digestion products						gel extraction			column			no purification
ligase used						L1	L2	L3	L1	L2	L3	L1
amount loaded	10 µL	0,5 µL	1 µL	2 µL	3 µL	10 µL						3 µL	10 µL

@@ Line 34: / Line 34: @@
 |rowspan="3"|D202
 |rowspan="3"|D202
-|1
+|'''1'''
-|2
+|'''2'''
-|3
+|'''3'''
-|4
+|'''4'''
-|5
+|'''5'''
-|6
+|'''6'''
-|7
+|'''7'''
 |rowspan="3"|100 bp ladder
 |-