Team:Paris/July 13

(Difference between revisions)

Latest revision as of 08:52, 16 July 2008

Dilution 1/200e (1mL of the O/N culture on 200mL of LB)
Culture at 37°C until DO=0,57 (around 3h)
Sharing into 4 Falcon tubes of 50mL and cooling at 4°C during 40' in ice
Spin during 8', 2000rpm, 4°C
Resuspension of the cell's pellet in 20mL of cold CaCl₂ (0,1M) during 30' in ice
Spin during 8', 2000rpm, 4°C
Resuspension of the cell's pellet in 1mL of cold CaCl₂ (0,1M)
1h of delay unexpected (problem with DNA lyophilized)
Transformation of the Biobricks with the "classical" protocol (--> heat shock at 42°C during 40")
Incubation O/N at 37°C of the transformed bacteria

@@ Line 1: / Line 1: @@
 {{Paris/Calendar_Links|July 12|July 14}}
-[[Preparation of Competent Cells]]
+==Preparation of Competent Cells==
 * Dilution 1/200e (1mL of the O/N culture on 200mL of LB)
@@ Line 15: / Line 15: @@
-[[Remark :]]
+==Remark==
 * Biobricks 2007 : use of 3µl of the minipreps
@@ Line 22: / Line 22: @@
-[[Liste of the transformation:]]
+==List of the transformations==
 *'''Biobricks 2007'''
@@ Line 145: / Line 145: @@
-[[Stock solution :]]
+==Stock solution==
 *TE (10:1) pH 8.0
 *plates of Ampicilin and Kanamycin