Team:Paris/July 14

Results of the transformation

• Positive control : pUC19 (1µl; 10pg/ml) --> No transformation
• Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice
• Biobricks 2008 : --> No transformation

          Conclusion : Cells are competents, because there is positive transformation
          Hypothesis : problem with the protocol of DNA extraction from the registery 2008

Culture of Cells

• Culture of DH5ɑ in 5ml of LB without Antibiotics, O/N at 37°C

Preparation of medium and solutions

• 1L of CCMB80
• 500ml of SOB ( 2 x 250ml)
• 500ml of SOC (2 x 250ml)
• 100ml of MgCl₂ (1M)
• 150ml of CaCl₂ (1M)
• 200ml of KOAC (1M)