Team:Paris/July 17

Results of the transformations

Legende :

• few , medium, a lot --> description of the number of CFU(Colony forming unit) produce by the transformation
• A: Ampicilin, K: Kanmycin

Cloning of the transformations

• Test 8 clones by Biobricks
• Incubate O/N at 37°C on proper plates

Screening PCR of the transformations

Mix for PCR (x76 tubes)

25µl Quick load 2x 1µl Oligo F 1µl Oligo R 23µl H2O

Protocol

• 50µl of Mix PCR by tubes
• 1 clone
• Programme : 105°C - for 28 cycles : 95°C; 5' - 95°C; 30" - 55°C; 30" - 72°C; 1,5'