Team:Paris/July 18

From 2008.igem.org

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(→Chosen Clones)
(→Analysis of the Electrophoresis)
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==Analysis of the Electrophoresis==
==Analysis of the Electrophoresis==
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{| border="1"
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Analyze here !
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|align="center"|'''Biobricks'''
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|align="center"|'''Clone'''
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|align="center"|'''Expected size'''
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|align="center"|'''Measured size'''
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|-
 +
|align="left"|E1010
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|align="center"|1
 +
|align="center"|5.106 kb
 +
|align="center"|~6-8 kb
 +
|-
 +
|align="left"|E1010
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|align="center"|2
 +
|align="center"|5.106 kb
 +
|align="center"|~6-8 kb
 +
|-
 +
|align="left"|C0079
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|align="center"|1
 +
|align="center"|5.181 kb
 +
|align="center"|~6-8 kb
 +
|-
 +
|align="left"|C0079
 +
|align="center"|2
 +
|align="center"|5.181 kb
 +
|align="center"|~6-8 kb
 +
|-
 +
|align="left"|C0079
 +
|align="center"|3
 +
|align="center"|5.181 kb
 +
|align="center"|~6-8 kb
 +
|-
 +
|align="left"|J23101 : problem !!!
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|align="center"|1
 +
|align="center"|3.037 kb
 +
|align="center"|~6-8 kb
 +
|-
 +
|align="left"|J23101 : problem !!!
 +
|align="center"|2
 +
|align="center"|3.037 kb
 +
|align="center"|~6-8 kb
 +
|-
 +
|align="left"|J23101 : problem !!!
 +
|align="center"|3
 +
|align="center"|3.037 kb
 +
|align="center"|~6-8 kb
 +
|-
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|align="left"|B0034
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|align="center"|2
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|align="center"|2.091 kb
 +
|align="center"|~2 kb
 +
|-
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|align="left"|B0034
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|align="center"|X
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|align="center"|2.091 kb
 +
|align="center"|~2 kb
 +
|-
 +
|align="left"|B0034 : problem !!!
 +
|align="center"|Y
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|align="center"|2.091 kb
 +
|align="center"|~5 kb
 +
|-
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|align="left"|E0040
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|align="center"|1
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|align="center"|2.799 kb
 +
|align="center"|~2.5-3 kb
 +
|-
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|align="left"|E0040
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|align="center"|2
 +
|align="center"|2.799 kb
 +
|align="center"|~2.5-3 kb
 +
|-
 +
|align="left"|R0040
 +
|align="center"|?
 +
|align="center"|2.133 kb
 +
|align="center"|~2 kb
 +
|}
==Culture of chosen clones carrying BioBricks==
==Culture of chosen clones carrying BioBricks==

Revision as of 13:04, 21 July 2008

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Contents

Analysis of the Electrophoresis

Biobricks Clone Expected size Measured size
E1010 1 5.106 kb ~6-8 kb
E1010 2 5.106 kb ~6-8 kb
C0079 1 5.181 kb ~6-8 kb
C0079 2 5.181 kb ~6-8 kb
C0079 3 5.181 kb ~6-8 kb
J23101 : problem !!! 1 3.037 kb ~6-8 kb
J23101 : problem !!! 2 3.037 kb ~6-8 kb
J23101 : problem !!! 3 3.037 kb ~6-8 kb
B0034 2 2.091 kb ~2 kb
B0034 X 2.091 kb ~2 kb
B0034 : problem !!! Y 2.091 kb ~5 kb
E0040 1 2.799 kb ~2.5-3 kb
E0040 2 2.799 kb ~2.5-3 kb
R0040 ? 2.133 kb ~2 kb

Culture of chosen clones carrying BioBricks

Chosen Clones

Biobricks Antibio Chosen Clones
B0034 A 1, 2 & 3
R0079 A 3, 4 & 6
C0079 K 1, 2 & 3
C0179 A 1, 2 & 7
J23101 A 1, 2 & 3
J23109 A 1, 2 & 5
R0040 A 1 & 2
pSB1A3 A 1, 2 & 3
E1010 K 1, 2 & 3
E0040 A 1 & 2
E0030 K 1, 2 & 3

Overnight culture of selected clones

Culture of the selected clones 5 mL of LB media containing the apropriate antibiotic. Incubation at 37°C overnight.

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