From 2008.igem.org
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Revision as of 16:29, 25 July 2008
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MiniPreps
- The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 24th.
| Name
| Biobrick
| Description
|
| MP100
| B0034
|
|
| MP116
| J23100
|
|
| MP120
| B0030
|
|
| MP121
| E0422
|
|
| MP122
| E0840
|
|
Digestion
Digestion Mix
10µl of Miniprep
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2
list of digestion
| Name
| Miniprep
| Biobrick
| Enzyme 1
| Enzyme 2
| Nber tube
|
| D100
| MP100
| B0034
| XbaI
| PstI
| 2
|
|
|
|
| EcoRI
| XbaI
| 1
|
|
|
|
| SpeI
| PstI
| 2
|
| D116
| MP116
| J23100
| SpeI
| PstI
| 2
|
| D120
| MP120
| B0030
| SpeI
| PstI
| 2
|
|
|
|
| EcoRI
| XbaI
| 1
|
|
|
|
| XbaI
| PstI
| 2
|
| D121
| MP121
| E0422
| XbaI
| PstI
| 1 !
|
| D122
| MP122
| E0840
| XbaI
| PstI
| 1 !
|
Results of digestions : Electrophoresis
| Name
| BioBrick
| Tube N°
| Enz 1
| Enz 2
| Obs
| Exp Size Matrix
| Exp Size BB
| Mea Size Matrix
| Mea Size BB
|
| D100
| B0034
| 1
| XbaI
| PstI
| BI
| 2057 pb
| 34 pb
|
|
|
| 2
|
|
|
| 3
| EcoRI
| XbaI
| FV
| 2076 pb
| 15 pb
|
|
|
| 4
| SpeI
| PstI
| BV
| 2077 pb
| 14 pb
|
|
|
| 5
|
|
|
| D101
| J23101
| 1
| SpeI
| PstI
| BV
| 2100 pb
| 883 pb
|
|
|
| 2
|
|
|
| D102
| J23109
| 1
| SpeI
| PstI
| BV
| 2100 pb
| 883 pb
|
|
|
| D103
| R0079
| 1
| SpeI
| PstI
| BV
| 2222 pb
| 14 pb
|
|
|
| 2
|
|
|
| D104
| R0040
| 1
| SpeI
| PstI
| BV
| 2119 pb
| 14 pb
|
|
|
| 2
|
|
|
| D105
| S03154
| 1
| SpeI
| PstI
| BV
| 2750 pb
| 14 pb
|
|
|
| 2
| XbaI
| PstI
|
| 2057 pb
| 707 pb
|
|
|
| 3
|
|
|
| 4
| EcoRI
| SpeI
|
| 2056 pb
| 708 pb
|
|
|
| D106
| S03879
| 1
| SpeI
| PstI
| BV
| 2768 pb
| 14 pb
|
|
|
| 2
| XbaI
| PstI
|
| 2057 pb
| 725 pb
|
|
|
| 3
|
|
|
| 4
| EcoRI
| SpeI
|
| 2756 pb
| 726 pb
|
|
|
| D107
| C0079
| 1
| EcoRI
| SpeI
| FI
| 4402 pb
| 779 pb
|
|
|
| 2
| XbaI
| PstI
| BI
| 4003 pb
| 778 pb
|
|
|
| D108
| C0179
| 1
| EcoRI
| SpeI
| FI
| 4402 pb
| 746 pb
|
|
|
| 2
| XbaI
| PstI
| BI
| 4403 pb
| 745 pb
|
|
|
| D109
| E0030
| 1
| EcoRI
| SpeI
| FI
| 3166 pb
| 746 pb
|
|
|
| 2
| XbaI
| PstI
| BI
| 3167 pb
| 745 pb
|
|
|
| D110
| E0040
| 1
| EcoRI
| SpeI
| FI
| 2056 pb
| 743 pb
|
|
|
| 2
| XbaI
| PstI
| BI
| 2057 pb
| 742 pb
|
|
|
| D111
| E1010
| 1
| EcoRI
| SpeI
| FI
| 4402 pb
| 704 pb
|
|
|
| 2
| XbaI
| PstI
| BI
| 4403 pb
| 703 pb
|
|
|
| D116
| J23100
| 1
| SpeI
| PstI
| BV
| 2100 pb
| 883 pb
|
|
|
| D117
| J23107
| 1
| SpeI
| PstI
| BV
| 2100 pb
| 883 pb
|
|
|
| D118
| B0015
| 1
| EcoRI
| XbaI
| FV
| 3303 pb
| 15 pb
|
|
|
| D119
| I0500
| 1
| SpeI
| PstI
| FV
| 5621 pb
| 14 pb
|
|
|
| D120
| B0030
| 1
| XbaI
| PstI
| BI
| 2057 pb
| 37 pb
|
|
|
| 2
|
|
|
| 3
| EcoRI
| XbaI
| FV
| 2079 pb
| 15 pb
|
|
|
| 4
| SpeI
| PstI
| BV
| 2080 pb
| 14 pb
|
|
|
| 5
|
|
|
| D121
| E0422
| 1
| XbaI
| PstI
| FV
| 2057 pb
| 939 pb
|
|
|
| D122
| E0840
| 1
| XbaI
| PstI
| FV
| 2057 pb
| 900 pb
|
|
|
|
"
