Team:Paris/August 1

From 2008.igem.org

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{{Paris/Calendar_Links|July 31|August 2}}
{{Paris/Calendar_Links|July 31|August 2}}
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minipreps
 
souche stockage
souche stockage
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* Use of Promega's protocol on all the clones cultivated on the 24th.
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* Use of Quiagen's protocol on all the clones cultivated on the 31th.
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* Preparation of 50µl of minipreps in simple.
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* Preparation of 50µl of minipreps in water.
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|align="center"|'''Description'''
|align="center"|'''Description'''
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|-
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|align="center"|MP100
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|align="center"|MP L102
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|align="center"|[http://partsregistry.org/Part:BBa_B0034 B0034]
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|align="center"|
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|align="center"|Strongest RBS (Efficiency = 1)
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|align="center"|
|-
|-
 +
|align="center"|MP L103
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|align="center"|
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|align="center"|
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|-
 +
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|align="center"|MP L104
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L105
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L106
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L107
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L108
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L110
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L111
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L112
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L115
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L116
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L117
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L118
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L119
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L121
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L122
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|align="center"|
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|align="center"|
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|-
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|align="center"|MP L124
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|align="center"|
 +
|align="center"|
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|-
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|align="center"|MP L125
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|align="center"|
 +
|align="center"|
 +
|}
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Revision as of 12:08, 4 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

souche stockage


Contents

MiniPreps

  • Use of Quiagen's protocol on all the clones cultivated on the 31th.
  • Preparation of 50µl of minipreps in water.


List of Minipreps

Name Biobrick Description
MP L102
MP L103
MP L104
MP L105
MP L106
MP L107
MP L108
MP L110
MP L111
MP L112
MP L115
MP L116
MP L117
MP L118
MP L119
MP L121
MP L122
MP L124
MP L125


Transformations

Protocol

use of TOP10 Chemically competent cells

  • Defroze competent cells on ice during 5'
  • Add 5µl of DNA Ligation in 50µL of competent bacterias (or 1µL for the positive control puc19)
  • Incubate 30' on ice
  • Heat-shock the cells during 30" at 42°C without shaking
  • Put 2' on ice
  • Add 250µL of pre-warmed SOC medium (4°C)
  • Incubate 1h at 37°C under shaking (225rpm)
  • Spin at 5.000rpm during 30"
  • Remove 150µL of supernatant
  • Resuspent the pellet in the 150µL left
  • Spread on adequated plates
  • Incubate O/N at 37°C


List of transformation

Name Biobrick Description
L100
L101
L113
L114
L120
L122
L123
L126
Retrieved from "http://2008.igem.org/Team:Paris/August_1"