Team:Paris/August 1

From 2008.igem.org

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|align="center"|L100
|align="center"|L100
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|align="center"| RBS-TetR-ECFP
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|align="center"|L101
|align="center"|L101
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|align="center"|RBS-tetR-GFP tripart
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|align="center"|L113
|align="center"|L113
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|align="center"|pBAD-ECFP
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|align="center"|L114
|align="center"|L114
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|align="center"|pBAD-GFP
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|align="center"|L120
|align="center"|L120
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|align="center"|tetR-ECFP
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|align="center"|L122
|align="center"|L122
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|align="center"|RBS-lasI-ECFP
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|align="center"|L123
|align="center"|L123
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|align="center"|RBS-lasI-GFP-ter
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|align="center"|L126
|align="center"|L126
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|align="center"|RBS-lasR-LVA
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Revision as of 12:18, 4 August 2008

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Contents

MiniPreps

Conditions

  • Use of Quiagen's protocol on all the clones cultivated on the 31th.
  • Preparation of 50µl of minipreps in water.


List of Minipreps

Name Biobrick Description
MP L102
MP L103
MP L104
MP L105
MP L106
MP L107
MP L108
MP L110
MP L111
MP L112
MP L115
MP L116
MP L117
MP L118
MP L119
MP L121
MP L122
MP L124
MP L125

Transformations

Protocol

use of TOP10 Chemically competent cells

  • Defroze competent cells on ice during 5'
  • Add 5µl of DNA Ligation in 50µL of competent bacterias (or 1µL for the positive control puc19)
  • Incubate 30' on ice
  • Heat-shock the cells during 30" at 42°C without shaking
  • Put 2' on ice
  • Add 250µL of pre-warmed SOC medium (4°C)
  • Incubate 1h at 37°C under shaking (225rpm)
  • Spin at 5.000rpm during 30"
  • Remove 150µL of supernatant
  • Resuspent the pellet in the 150µL left
  • Spread on adequated plates
  • Incubate O/N at 37°C


List of transformation

Name Biobrick Description
L100 RBS-TetR-ECFP
L101 RBS-tetR-GFP tripart
L113 pBAD-ECFP
L114 pBAD-GFP
L120 tetR-ECFP
L122 RBS-lasI-ECFP
L123 RBS-lasI-GFP-ter
L126 RBS-lasR-LVA
Retrieved from "http://2008.igem.org/Team:Paris/August_1"