Team:Paris/July 30

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Revision as of 13:54, 4 August 2008

Analysis of yesterday DNA digestion

The digested DNA of yesterday was analysed one more time by electrophoresis on a 0.8% agarose gel (about 30 minutes at 100 W). The ladder used was the 1 kb DNA ladder (New England Biolabs). 5 µL of each sample with 1 µL of loading dye were loaded.

D101	B0034	1	EcoRI	XbaI	FV	2076 pb	2000 pb		5
Name	BioBrick	Tube N°	Enz 1	Enz 2	Obs	Exp Size	Mea Size	Conc (ng/µl)	Band
D102	B0034	1	SpeI	PstI	BV	2077 pb			11
D105	R0079	1	SpeI	PstI	BV	2222 pb			13
D106	R0040	1	SpeI	PstI	BV	2119 pb			12
D108	S03154	1	XbaI	PstI	BI	707 pb		0 (failed)	14
D111	S03879	1	XbaI	PstI	BI	725 pb			15
D115	C0179	2	EcoRI	SpeI	FI	746 pb			4 & 5
D116	C0179	2	XbaI	PstI	BI	745 pb			2 & 3
D117	E0030	1	EcoRI	SpeI	FI	746 pb			16
D128	B0030	2	EcoRI	XbaI	FV	2079 pb			6 & 7
D129	B0030	2	SpeI	PstI	BV	2080 pb			8 & 9

Results : Each of the samples was succesfully digested and purified except for the sample D108. It seems that the QIAprep columms (from the QIAGEN Minipreps kit) can be used instead of the QIAquick columms (for DNA Gel Extraction).

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 ! rowspan="1"| 2076 pb
 ! rowspan="1"| 2000 pb
-! rowspan="1"|
 ! rowspan="1"|
 ! rowspan="1"| 5