Team:Paris/August 2

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Revision as of 16:27, 4 August 2008

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Results of the transformation

Ligation name

Description

Antibio

Nb colonies observed

Fluorescence

L100

rbs TetR - ECFP

D110 (BV) - D130 (BI)

-

L101

rbs TetR - GFP tripart

D110 (BV) - D131 (BI)

-

L102

Strong rbs - YFP

D129 (BV) - D118 (BI)

-

L103

Strong rbs - GFP

D129 (BV) - D122 (BI)

-

L104

Strong rbs - lasR activator

D129 (BV) - D114 (BI)

L105

Strong promoter - ECFP
D123 (BV) - D130 (BI)

L106

Strong promoter - gfp Tripart
D123 (BV) - D131 (BI)

L107

Strongest promoter - ECFP
D103 (BV) - D131 (BI)

L108

Strong promoter - gfp Tripart
D103 (BV) - D130 (BI)

L110

Medium promoter - gfp Tripart
D124 (BV) - D131 (BI)

L111

Weak promoter - ECFP
D104 (BV) - D130 (BI)

L112

Weak promoter - gfp
D104 (BV) - D131 (BI)

L115

pLas - ECFP
D105 (BV) - D130 (BI)

L116

pLas - gfp Tripart
D105 (BV) - D131 (BI)

L117

yfp - Double Terminator
D125 (FV) - D117 (FI)

L118

rfp - Double Terminator
D125 (FV) - D121 (FI)

L119

lasR activator + LVA - Double Terminator
D125 (FV) - D113 (FI)

L121

Strong promoter - gfp tripart
D106 (BV) - D130 (BI)

L124

Strongest RBS - rfp
D102 (BV) - D122 (BI)

L125

Strongest RBS - yfp
D102 (BV) - D118 (BI)

@@ Line 11: / Line 11: @@
 |align="center"|'''Fluorescence'''
 |-
-|align="center"|puc19
+|align="center"|L100
-|align="center"|positive control
+|align="center"|rbs TetR - ECFP
-|align="center"|A
+<br>D110 (BV) -  D130 (BI)
-|align="center"| CFU
 |align="center"|
-|-
+|align="center"|
+|align="center"|
+-
+|align="center"|L101
+|align="center"|rbs TetR - GFP tripart
+<br>D110 (BV) -  D131 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+-
+|align="center"|L102
+|align="center"|Strong rbs - YFP
+<br>D129 (BV) -  D118 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+-
+|align="center"|L103
+|align="center"|Strong rbs - GFP
+<br>D129 (BV) -  D122 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+-
 |align="center"|L104
 |align="center"|Strong rbs - lasR activator
@@ Line 98: / Line 120: @@
 |align="center"|L117
 |align="center"|
-Double Terminator - yfp
+yfp - Double Terminator
 <br>D125 (FV) - D117 (FI)
 |align="center"|
@@ Line 106: / Line 128: @@
 |align="center"|L118
 |align="center"|
-Double Terminator - rfp
+rfp - Double Terminator
 <br>D125 (FV) - D121 (FI)
 |align="center"|
@@ Line 114: / Line 136: @@
 |align="center"|L119
 |align="center"|
-Double Terminator - lasR activator + LVA
+lasR activator + LVA - Double Terminator
 <br>D125 (FV) - D113 (FI)
 |align="center"|
@@ Line 143: / Line 165: @@
 |align="center"|
 |align="center"|
-|align="center"|
-|align="center"|L100
-|align="center"|
-|align="center"|A
-|align="center"|0 CFU
-|align="center"|0 CFU
-|-
-|align="center"|L113
-|align="center"|
-|align="center"|K
-|align="center"|few
-|align="center"|
-|-
-|align="center"|L114
-|align="center"|
-|align="center"|K
-|align="center"|medium
-|align="center"|
-|-
-|align="center"|L120
-|align="center"|
-|align="center"|A
-|align="center"|few
-|align="center"|
-|-
-|align="center"|L122
-|align="center"|
-|align="center"|A
-|align="center"|medium
-|align="center"|
-|-
-|align="center"|L123
-|align="center"|
-|align="center"|A
-|align="center"|0 CFU
-|align="center"|
-|-
-|align="center"|L126
-|align="center"|
-|align="center"|A
-|align="center"|a lot
 |align="center"|
 |}