Team:Paris/August 2

From 2008.igem.org

(Difference between revisions)
(→Results of the transformation)
(→Results of the transformation)
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|align="center"|'''Nb colonies observed'''
|align="center"|'''Nb colonies observed'''
|align="center"|'''Fluorescence'''
|align="center"|'''Fluorescence'''
 +
|align="center"|'''Comments'''
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|align="center"|L100
|align="center"|L100
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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|align="center"|L101
|align="center"|L101
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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|align="center"|L102
|align="center"|L102
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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|align="center"|L103
|align="center"|L103
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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|align="center"|L104
|align="center"|L104
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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|align="center"|L105
|align="center"|L105
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|OK
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|align="center"|L106
|align="center"|L106
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|OK
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|align="center"|L107
|align="center"|L107
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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|-
-
|align="center"|L108
+
|align="center"|L108 n°2 (the right one)
|align="center"|
|align="center"|
Strong promoter -  gfp Tripart
Strong promoter -  gfp Tripart
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|align="center"|
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|align="center"|
 +
|align="center"|OK
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|align="center"|L110
|align="center"|L110
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|OK
|-
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|align="center"|L111
|align="center"|L111
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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|align="center"|L112
|align="center"|L112
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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|align="center"|L115
|align="center"|L115
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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|align="center"|L116
|align="center"|L116
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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|align="center"|L117
|align="center"|L117
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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|align="center"|L118
|align="center"|L118
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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|align="center"|L119
|align="center"|L119
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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|-
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|align="center"|L120
 +
|align="center"|
 +
tetR repressible - ECFP
 +
<br>D106 (BV) - D130 (BI)
 +
|align="center"|
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|align="center"|
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|align="center"|
 +
|align="center"|To do again
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|align="center"|L121
|align="center"|L121
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|OK
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|-
 +
|align="center"|L122
 +
|align="center"|
 +
RBS-lasI - ecfp
 +
<br>D107 (BV) - D130 (BI)
 +
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 +
|align="center"|
 +
|align="center"|
 +
|align="center"|To do again
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|-
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|align="center"|L123
 +
|align="center"|
 +
RBS lasI - ecfp
 +
<br>D107 (BV) - D131 (BI)
 +
|align="center"|
 +
|align="center"|
 +
|align="center"|
 +
|align="center"|To do again
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|align="center"|L124
|align="center"|L124
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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|align="center"|L125
|align="center"|L125
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|align="center"|
|align="center"|
|align="center"|
 +
|align="center"|To do again
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Revision as of 16:42, 4 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Results of the transformation

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
L100 rbs TetR - ECFP


D110 (BV) - D130 (BI)

To do again
L101 rbs TetR - GFP tripart


D110 (BV) - D131 (BI)

To do again
L102 Strong rbs - YFP


D129 (BV) - D118 (BI)

To do again
L103 Strong rbs - GFP


D129 (BV) - D122 (BI)

To do again
L104 Strong rbs - lasR activator


D129 (BV) - D114 (BI)

To do again
L105

Strong promoter - ECFP
D123 (BV) - D130 (BI)

OK
L106

Strong promoter - gfp Tripart
D123 (BV) - D131 (BI)

OK
L107

Strongest promoter - ECFP
D103 (BV) - D131 (BI)

To do again
L108 n°2 (the right one)

Strong promoter - gfp Tripart
D103 (BV) - D130 (BI)

OK
L110

Medium promoter - gfp Tripart
D124 (BV) - D131 (BI)

OK
L111

Weak promoter - ECFP
D104 (BV) - D130 (BI)

To do again
L112

Weak promoter - gfp
D104 (BV) - D131 (BI)

To do again
L115

pLas - ECFP
D105 (BV) - D130 (BI)

To do again
L116

pLas - gfp Tripart
D105 (BV) - D131 (BI)

To do again
L117

yfp - Double Terminator
D125 (FV) - D117 (FI)

To do again
L118

rfp - Double Terminator
D125 (FV) - D121 (FI)

To do again
L119

lasR activator + LVA - Double Terminator
D125 (FV) - D113 (FI)

To do again
L120

tetR repressible - ECFP
D106 (BV) - D130 (BI)

To do again
L121

Strong promoter - gfp tripart
D106 (BV) - D130 (BI)

OK
L122

RBS-lasI - ecfp
D107 (BV) - D130 (BI)

To do again
L123

RBS lasI - ecfp
D107 (BV) - D131 (BI)

To do again
L124

Strongest RBS - rfp
D102 (BV) - D122 (BI)

To do again
L125

Strongest RBS - yfp
D102 (BV) - D118 (BI)

To do again
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