Team:Paris/August 2

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(Difference between revisions)

Revision as of 17:23, 4 August 2008

Results of the transformation

Ligation name	Description	Antibio	Nb colonies observed	Fluorescence	Comments
L100	rbs TetR - ECFP D110 (BV) - D130 (BI)				To do again
L101	rbs TetR - GFP tripart D110 (BV) - D131 (BI)				To do again
L102	Strong rbs - YFP D129 (BV) - D118 (BI)				To do again
L103	Strong rbs - GFP D129 (BV) - D122 (BI)				To do again
L104	Strong rbs - lasR activator D129 (BV) - D114 (BI)				To do again
L105	Strong promoter - ECFP D123 (BV) - D130 (BI)				OK
L106	Strong promoter - gfp Tripart D123 (BV) - D131 (BI)				OK
L107	Strongest promoter - ECFP D103 (BV) - D131 (BI)				To do again
L108 n°2 (the right one)	Strong promoter - gfp Tripart D103 (BV) - D130 (BI)				OK
L110	Medium promoter - gfp Tripart D124 (BV) - D131 (BI)				OK
L111	Weak promoter - ECFP D104 (BV) - D130 (BI)				To do again
L112	Weak promoter - gfp D104 (BV) - D131 (BI)				To do again
L113	AracpBAD - ecfp D126 (BV) - D130 (BI)				To do again
L114	AracpBAD - gfp tripart D126 (BV) - D131 (BI)				To do again
L115	pLas - ECFP D105 (BV) - D130 (BI)				To do again
L116	pLas - gfp Tripart D105 (BV) - D131 (BI)				To do again
L117	yfp - Double Terminator D125 (FV) - D117 (FI)				To do again
L118	rfp - Double Terminator D125 (FV) - D121 (FI)				To do again
L119	lasR activator + LVA - Double Terminator D125 (FV) - D113 (FI)				To do again
L120	tetR repressible - ECFP D106 (BV) - D130 (BI)				To do again
L121	Strong promoter - gfp tripart D106 (BV) - D130 (BI)				OK
L122	RBS-lasI - ecfp D107 (BV) - D130 (BI)				To do again
L123	RBS lasI - ecfp D107 (BV) - D131 (BI)				To do again
L124	Strongest RBS - rfp D102 (BV) - D122 (BI)				To do again
L125	Strongest RBS - yfp D102 (BV) - D118 (BI)				To do again	L126	Strongest RBS (1)- LacR activator (+LVA) D102 (BV) - D118 (BI)	To do again

Results of the transformation (bis)

Ligation name	Description	Antibio	Nb colonies observed	Fluorescence	Comments
L100	rbs TetR - ECFP D110 (BV) - D130 (BI)		608		To do again
L101	rbs TetR - GFP tripart D110 (BV) - D131 (BI)		> 4000		To do again
L113	AracpBAD - ecfp D126 (BV) - D130 (BI)		9		To do again
L114	AracpBAD - gfp tripart D126 (BV) - D131 (BI)		9		To do again
L120	tetR repressible - ECFP D106 (BV) - D130 (BI)		74		To do again
L122	RBS-lasI - ecfp D107 (BV) - D130 (BI)		4		To do again
L123	RBS lasI - ecfp D107 (BV) - D131 (BI)		> 4000		To do again
L126	Strongest RBS (1)- LacR activator (+LVA) D102 (BV) - D118 (BI)		944		To do again

Results of the transformation bis the right one

Ligation name	Description	Antibio	Nb colonies observed	Fluorescence	Comments
L100	rbs TetR - ECFP D110 (BV) - D130 (BI)				To do again
L101	rbs TetR - GFP tripart D110 (BV) - D131 (BI)				To do again
L113	AracpBAD - ecfp D126 (BV) - D130 (BI)				To do again
L114	AracpBAD - gfp tripart D105 (BV) - D130 (BI)				To do again
L120	tetR repressible - ECFP D106 (BV) - D130 (BI)				To do again
L122	RBS-lasI - ecfp D107 (BV) - D130 (BI)				To do again
L123	RBS lasI - ecfp D107 (BV) - D131 (BI)				To do again
L126	Strongest RBS - lacR activator (+LVA) D102 (BV) - D122 (BI)				To do again

@@ Line 110: / Line 110: @@
 Weak promoter - gfp
 <br>D104 (BV) - D131 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|align="center"|To do again
+|-
+|align="center"|L113
+|align="center"|
+AracpBAD - ecfp
+<br>D126 (BV) - D130 (BI)
+|align="center"|
+|align="center"|
+|align="center"|
+|align="center"|To do again
+|-
+|align="center"|L114
+|align="center"|
+AracpBAD - gfp tripart
+<br>D126 (BV) - D131 (BI)
 |align="center"|
 |align="center"|
@@ Line 220: / Line 238: @@
 |align="center"|
 |align="center"|
+|align="center"|
+|align="center"|To do again
+|}
+=== Results of the transformation (bis)===
+{| border="1"
+|align="center"|'''Ligation name'''
+|align="center"|'''Description'''
+|align="center"|'''Antibio'''
+|align="center"|'''Nb colonies observed'''
+|align="center"|'''Fluorescence'''
+|align="center"|'''Comments'''
+|-
+|align="center"|L100
+|align="center"|rbs TetR - ECFP
+<br>D110 (BV) -  D130 (BI)
+|align="center"|
+|align="center"|608
+|align="center"|
+|align="center"|To do again
+|-
+|align="center"|L101
+|align="center"|rbs TetR - GFP tripart
+<br>D110 (BV) -  D131 (BI)
+|align="center"|
+|align="center"|> 4000
+|align="center"|
+|align="center"|To do again
+|-
+|align="center"|L113
+|align="center"|
+AracpBAD - ecfp
+<br>D126 (BV) - D130 (BI)
+|align="center"|
+|align="center"|9
+|align="center"|
+|align="center"|To do again
+|-
+|align="center"|L114
+|align="center"|
+AracpBAD - gfp tripart
+<br>D126 (BV) - D131 (BI)
+|align="center"|
+|align="center"|9
+|align="center"|
+|align="center"|To do again
+|-
+|align="center"|L120
+|align="center"|
+tetR repressible - ECFP
+<br>D106 (BV) - D130 (BI)
+|align="center"|
+|align="center"|74
+|align="center"|
+|align="center"|To do again
+|-
+|align="center"|L122
+|align="center"|
+RBS-lasI - ecfp
+<br>D107 (BV) - D130 (BI)
+|align="center"|
+|align="center"|4
+|align="center"|
+|align="center"|To do again
+|-
+|align="center"|L123
+|align="center"|
+RBS lasI - ecfp
+<br>D107 (BV) - D131 (BI)
+|align="center"|
+|align="center"|> 4000
+|align="center"|
+|align="center"|To do again
+|-
+|align="center"|L126
+|align="center"|
+Strongest RBS (1)- LacR activator (+LVA)
+<br>D102 (BV) - D118 (BI)
+|align="center"|
+|align="center"|944
 |align="center"|
 |align="center"|To do again