Team:Paris/August 2

From 2008.igem.org

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|align="center"|To do again
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|align="center"|'''Controls'''
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|align="center"|C1
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D110
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|align="center"|0
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|align="center"|C2
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D129
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|align="center"|5
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|align="center"|C3
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D123
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|align="center"|+/- 100
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|align="center"|C4
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D103
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|align="center"|+/- 20
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|align="center"|C5
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D124
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|align="center"|+/- 100
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|align="center"|C6
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D104
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|align="center"|+/- 100
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|align="center"|C7
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D126
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|align="center"|0
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|align="center"|C8
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D105
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|align="center"|+/- 20
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|align="center"|C9
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D125
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|align="center"|0
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|align="center"|C10
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D106
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|align="center"|
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|align="center"|0
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|align="center"|C11
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|align="center"|
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D107
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|align="center"|
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|align="center"|0
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|align="center"|C12
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D102
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|align="center"|+/- 10
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|align="center"|Positive control
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puc19
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|align="center"|155 (transformation efficiency:1.5*10^7/ug)
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Revision as of 18:03, 4 August 2008

← Yesterday

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Results of the transformation

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
L100

rbs TetR - ECFP
D110 (BV) - D130 (BI)

0 To do again
L101

rbs TetR - GFP tripart
D110 (BV) - D131 (BI)

(1) To do again
L102

Strong rbs - YFP
D129 (BV) - D118 (BI)

+/- 100 To do again
L103

Strong rbs - GFP
D129 (BV) - D122 (BI)

a lot ++ To do again
L104

Strong rbs - lasR activator
D129 (BV) - D114 (BI)

a lot ++ To do again
L105

Strong promoter - ECFP
D123 (BV) - D130 (BI)

a lot ++ OK
L106

Strong promoter - gfp Tripart
D123 (BV) - D131 (BI)

a lot ++ OK
L107

Strongest promoter - ECFP
D103 (BV) - D131 (BI)

a lot ++ To do again
L108 n°2 (the right one)

Strong promoter - gfp Tripart
D103 (BV) - D130 (BI)

+/- 100 OK
L109

Strong promoter - ecfp
D124 (BV) - D130 (BI)

+/- 100 To do again
L110

Medium promoter - gfp Tripart
D124 (BV) - D131 (BI)

a lot ++ OK
L111

Weak promoter - ECFP
D104 (BV) - D130 (BI)

a lot ++ To do again
L112

Weak promoter - gfp
D104 (BV) - D131 (BI)

a lot ++ To do again
L113

AracpBAD - ecfp
D126 (BV) - D130 (BI)

1 To do again
L114

AracpBAD - gfp tripart
D126 (BV) - D131 (BI)

2 To do again
L115

pLas - ECFP
D105 (BV) - D130 (BI)

a lot ++ To do again
L116

pLas - gfp Tripart
D105 (BV) - D131 (BI)

a lot ++ To do again
L117

yfp - Double Terminator
D117 (FI) - D125 (FV)

+/- 30 To do again
L118

rfp - Double Terminator
D121 (FI) - D125 (FV)

+/- 30 To do again
L119

lasR activator + LVA - Double Terminator
D113 (FI) - D125 (FV)

+/- 30 To do again
L120

tetR repressible - ECFP
D106 (BV) - D130 (BI)

a lot ++ To do again
L121

Strong promoter - gfp tripart
D106 (BV) - D130 (BI)

a lot ++ OK
L122

RBS-lasI - ecfp
D107 (BV) - D130 (BI)

0 To do again
L123

RBS lasI - ecfp
D107 (BV) - D131 (BI)

1 To do again
L124

Strongest RBS - rfp
D102 (BV) - D122 (BI)

a lot ++ To do again
L125

Strongest RBS - yfp
D102 (BV) - D118 (BI)

a lot ++ To do again
L126

Strongest RBS (1)- LacR activator (+LVA)
D102 (BV) - D118 (BI)

no dish found!!! To do again
L127

gfp (1)- Double terminator
D119 (FI) - D125 (FV)

no dish found!!! To do again
Controls
C1

D110

0 -
C2

D129

5 -
C3

D123

+/- 100 -
C4

D103

+/- 20 -
C5

D124

+/- 100 -
C6

D104

+/- 100 -
C7

D126

0 -
C8

D105

+/- 20 -
C9

D125

0 -
C10

D106

0 -
C11

D107

0 -
C12

D102

+/- 10 -
Positive control

puc19

155 (transformation efficiency:1.5*10^7/ug) -

Results of the transformation (bis-the good one to leave in august 2)

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
L100

rbs TetR - ECFP
D110 (BV) - D130 (BI)

Amp 608 To do again
L101

rbs TetR - GFP tripart
D110 (BV) - D131 (BI)

Amp > 4000 GFP positive OK
L113

AracpBAD - ecfp
D126 (BV) - D130 (BI)

Kana 9 To do again
L114

AracpBAD - gfp tripart
D126 (BV) - D131 (BI)

Kana 9 To do again
L120

tetR repressible promoter - ECFP
D106 (BV) - D130 (BI)

Amp 74 To do again
L122

RBS-lasI - ecfp
D107 (BV) - D130 (BI)

Amp 4 To do again
L123

RBS lasI - ecfp
D107 (BV) - D131 (BI)

Amp > 4000 To do again
L126

Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)

Amp 944 To do again
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