Team:Paris/July 31

From 2008.igem.org

(Difference between revisions)
(→Results of the transformation)
(→Results of the transformation : Fluorescence)
Line 8: Line 8:
|align="center"|'''Ligation name'''
|align="center"|'''Ligation name'''
|align="center"|'''Description'''
|align="center"|'''Description'''
-
|align="center"|'''Antibio'''
 
-
|align="center"|'''Nb colonies observed'''
 
|align="center"|'''Fluorescence'''
|align="center"|'''Fluorescence'''
|align="center"|'''Comments'''
|align="center"|'''Comments'''
Line 18: Line 16:
<br>D110 (BV) -  D130 (BI)   
<br>D110 (BV) -  D130 (BI)   
|align="center"|
|align="center"|
-
|align="center"|0
 
-
|align="center"|
 
-
|align="center"|To do again
 
-
|-
 
-
|align="center"|L101
 
-
|align="center"|
 
-
rbs TetR - GFP tripart
 
-
<br>D110 (BV) -  D131 (BI) 
 
-
|align="center"|
 
-
|align="center"|(1)
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 35: Line 23:
Strong rbs - YFP
Strong rbs - YFP
<br>D129 (BV) -  D118 (BI)   
<br>D129 (BV) -  D118 (BI)   
-
|align="center"|
 
-
|align="center"|+/- 100
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 44: Line 30:
Strong rbs - GFP
Strong rbs - GFP
<br>D129 (BV) -  D122 (BI)   
<br>D129 (BV) -  D122 (BI)   
-
|align="center"|
 
-
|align="center"|a lot ++
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 53: Line 37:
Strong rbs - lasR activator
Strong rbs - lasR activator
<br>D129 (BV) -  D114 (BI)   
<br>D129 (BV) -  D114 (BI)   
-
|align="center"|
 
-
|align="center"|a lot ++
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 62: Line 44:
Strong promoter - ECFP
Strong promoter - ECFP
<br>D123 (BV) - D130 (BI)
<br>D123 (BV) - D130 (BI)
-
|align="center"|
 
-
|align="center"|a lot ++
 
|align="center"|
|align="center"|
|align="center"|OK
|align="center"|OK
Line 71: Line 51:
Strong promoter - gfp Tripart
Strong promoter - gfp Tripart
<br>D123 (BV) - D131 (BI)
<br>D123 (BV) - D131 (BI)
-
|align="center"|
 
-
|align="center"|a lot ++
 
|align="center"|
|align="center"|
|align="center"|OK
|align="center"|OK
Line 80: Line 58:
Strongest promoter - ECFP
Strongest promoter - ECFP
<br>D103 (BV) - D131 (BI)
<br>D103 (BV) - D131 (BI)
-
|align="center"|
 
-
|align="center"|a lot ++
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 89: Line 65:
Strong promoter -  gfp Tripart
Strong promoter -  gfp Tripart
<br>D103 (BV) - D130 (BI)
<br>D103 (BV) - D130 (BI)
-
|align="center"|
 
-
|align="center"|+/- 100
 
|align="center"|
|align="center"|
|align="center"|OK
|align="center"|OK
Line 98: Line 72:
Strong promoter -  ecfp
Strong promoter -  ecfp
<br>D124 (BV) - D130 (BI)
<br>D124 (BV) - D130 (BI)
-
|align="center"|
 
-
|align="center"|+/- 100
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 107: Line 79:
Medium promoter - gfp Tripart
Medium promoter - gfp Tripart
<br>D124 (BV) - D131 (BI)
<br>D124 (BV) - D131 (BI)
-
|align="center"|
 
-
|align="center"|a lot ++
 
|align="center"|
|align="center"|
|align="center"|OK
|align="center"|OK
Line 116: Line 86:
Weak promoter - ECFP
Weak promoter - ECFP
<br>D104 (BV) - D130 (BI)  
<br>D104 (BV) - D130 (BI)  
-
|align="center"|
 
-
|align="center"|a lot ++
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 125: Line 93:
Weak promoter - gfp
Weak promoter - gfp
<br>D104 (BV) - D131 (BI)
<br>D104 (BV) - D131 (BI)
-
|align="center"|
 
-
|align="center"|a lot ++
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 134: Line 100:
AracpBAD - ecfp
AracpBAD - ecfp
<br>D126 (BV) - D130 (BI)
<br>D126 (BV) - D130 (BI)
-
|align="center"|
 
-
|align="center"|1
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 143: Line 107:
AracpBAD - gfp tripart
AracpBAD - gfp tripart
<br>D126 (BV) - D131 (BI)
<br>D126 (BV) - D131 (BI)
-
|align="center"|
 
-
|align="center"|2
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 151: Line 113:
|align="center"|
|align="center"|
pLas - ECFP
pLas - ECFP
-
<br>D105 (BV) - D130 (BI)
 
-
|align="center"|
 
-
|align="center"|a lot ++
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 161: Line 120:
pLas - gfp Tripart
pLas - gfp Tripart
<br>D105 (BV) - D131 (BI)
<br>D105 (BV) - D131 (BI)
-
|align="center"|
 
-
|align="center"|a lot ++
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 170: Line 127:
yfp - Double Terminator
yfp - Double Terminator
<br>D117 (FI) - D125 (FV)
<br>D117 (FI) - D125 (FV)
-
|align="center"|
 
-
|align="center"|+/- 30
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 179: Line 134:
rfp - Double Terminator
rfp - Double Terminator
<br>D121 (FI) - D125 (FV)
<br>D121 (FI) - D125 (FV)
-
|align="center"|
 
-
|align="center"|+/- 30
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 188: Line 141:
lasR activator + LVA - Double Terminator
lasR activator + LVA - Double Terminator
<br>D113 (FI) - D125 (FV)
<br>D113 (FI) - D125 (FV)
-
|align="center"|
 
-
|align="center"|+/- 30
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 197: Line 148:
tetR repressible - ECFP
tetR repressible - ECFP
<br>D106 (BV) - D130 (BI)
<br>D106 (BV) - D130 (BI)
-
|align="center"|
 
-
|align="center"|a lot ++
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 206: Line 155:
Strong promoter - gfp tripart
Strong promoter - gfp tripart
<br>D106 (BV) - D130 (BI)
<br>D106 (BV) - D130 (BI)
-
|align="center"|
 
-
|align="center"|a lot ++
 
|align="center"|
|align="center"|
|align="center"|OK
|align="center"|OK
Line 215: Line 162:
RBS-lasI - ecfp
RBS-lasI - ecfp
<br>D107 (BV) - D130 (BI)
<br>D107 (BV) - D130 (BI)
-
|align="center"|
 
-
|align="center"|0
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 224: Line 169:
RBS lasI - ecfp
RBS lasI - ecfp
<br>D107 (BV) - D131 (BI)
<br>D107 (BV) - D131 (BI)
-
|align="center"|
 
-
|align="center"|1
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 233: Line 176:
Strongest RBS - rfp
Strongest RBS - rfp
<br>D102 (BV) - D122 (BI)
<br>D102 (BV) - D122 (BI)
-
|align="center"|
 
-
|align="center"|a lot ++
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 242: Line 183:
Strongest RBS - yfp
Strongest RBS - yfp
<br>D102 (BV) - D118 (BI)
<br>D102 (BV) - D118 (BI)
-
|align="center"|
 
-
|align="center"|a lot ++
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 251: Line 190:
Strongest RBS (1)- LacR activator (+LVA)
Strongest RBS (1)- LacR activator (+LVA)
<br>D102 (BV) - D118 (BI)
<br>D102 (BV) - D118 (BI)
-
|align="center"|
 
-
|align="center"|no dish found!!!
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 260: Line 197:
gfp (1)- Double terminator
gfp (1)- Double terminator
<br>D119 (FI) - D125 (FV)
<br>D119 (FI) - D125 (FV)
-
|align="center"|
 
-
|align="center"|no dish found!!!
 
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
|-
|-
|align="center"|'''Controls'''
|align="center"|'''Controls'''
-
|align="center"|
 
-
|align="center"|
 
|align="center"|
|align="center"|
|align="center"|
|align="center"|
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|align="center"|
|align="center"|
D110
D110
-
|align="center"|
 
-
|align="center"|0
 
|align="center"|
|align="center"|
|align="center"|-
|align="center"|-
Line 283: Line 214:
|align="center"|
|align="center"|
D129
D129
-
|align="center"|
 
-
|align="center"|5
 
|align="center"|
|align="center"|
|align="center"|-
|align="center"|-
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|align="center"|
|align="center"|
D123
D123
-
|align="center"|
 
-
|align="center"|+/- 100
 
|align="center"|
|align="center"|
|align="center"|-
|align="center"|-
Line 299: Line 226:
|align="center"|
|align="center"|
D103
D103
-
|align="center"|
 
-
|align="center"|+/- 20
 
|align="center"|
|align="center"|
|align="center"|-
|align="center"|-
Line 307: Line 232:
|align="center"|
|align="center"|
D124
D124
-
|align="center"|
 
-
|align="center"|+/- 100
 
|align="center"|
|align="center"|
|align="center"|-
|align="center"|-
Line 315: Line 238:
|align="center"|
|align="center"|
D104
D104
-
|align="center"|
 
-
|align="center"|+/- 100
 
|align="center"|
|align="center"|
|align="center"|-
|align="center"|-
Line 323: Line 244:
|align="center"|
|align="center"|
D126
D126
-
|align="center"|
 
-
|align="center"|0
 
|align="center"|
|align="center"|
|align="center"|-
|align="center"|-
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|align="center"|
|align="center"|
D105
D105
-
|align="center"|
 
-
|align="center"|+/- 20
 
|align="center"|
|align="center"|
|align="center"|-
|align="center"|-
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|align="center"|
|align="center"|
D125
D125
-
|align="center"|
 
-
|align="center"|0
 
|align="center"|
|align="center"|
|align="center"|-
|align="center"|-
Line 347: Line 262:
|align="center"|
|align="center"|
D106
D106
-
|align="center"|
 
-
|align="center"|0
 
|align="center"|
|align="center"|
|align="center"|-
|align="center"|-
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|align="center"|
|align="center"|
D107
D107
-
|align="center"|
 
-
|align="center"|0
 
|align="center"|
|align="center"|
|align="center"|-
|align="center"|-
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|align="center"|
|align="center"|
D102
D102
-
|align="center"|
 
-
|align="center"|+/- 10
 
|align="center"|
|align="center"|
|align="center"|-
|align="center"|-
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|align="center"|
|align="center"|
puc19
puc19
-
|align="center"|
 
-
|align="center"|155 (transformation efficiency:1.5*10^7/ug)
 
|align="center"|
|align="center"|
|align="center"|-
|align="center"|-
|}
|}

Revision as of 09:25, 5 August 2008

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Results of the transformation : Fluorescence

Ligation name Description Fluorescence Comments
L100

rbs TetR - ECFP
D110 (BV) - D130 (BI)

To do again
L102

Strong rbs - YFP
D129 (BV) - D118 (BI)

To do again
L103

Strong rbs - GFP
D129 (BV) - D122 (BI)

To do again
L104

Strong rbs - lasR activator
D129 (BV) - D114 (BI)

To do again
L105

Strong promoter - ECFP
D123 (BV) - D130 (BI)

OK
L106

Strong promoter - gfp Tripart
D123 (BV) - D131 (BI)

OK
L107

Strongest promoter - ECFP
D103 (BV) - D131 (BI)

To do again
L108 n°2 (the right one)

Strong promoter - gfp Tripart
D103 (BV) - D130 (BI)

OK
L109

Strong promoter - ecfp
D124 (BV) - D130 (BI)

To do again
L110

Medium promoter - gfp Tripart
D124 (BV) - D131 (BI)

OK
L111

Weak promoter - ECFP
D104 (BV) - D130 (BI)

To do again
L112

Weak promoter - gfp
D104 (BV) - D131 (BI)

To do again
L113

AracpBAD - ecfp
D126 (BV) - D130 (BI)

To do again
L114

AracpBAD - gfp tripart
D126 (BV) - D131 (BI)

To do again
L115

pLas - ECFP

To do again
L116

pLas - gfp Tripart
D105 (BV) - D131 (BI)

To do again
L117

yfp - Double Terminator
D117 (FI) - D125 (FV)

To do again
L118

rfp - Double Terminator
D121 (FI) - D125 (FV)

To do again
L119

lasR activator + LVA - Double Terminator
D113 (FI) - D125 (FV)

To do again
L120

tetR repressible - ECFP
D106 (BV) - D130 (BI)

To do again
L121

Strong promoter - gfp tripart
D106 (BV) - D130 (BI)

OK
L122

RBS-lasI - ecfp
D107 (BV) - D130 (BI)

To do again
L123

RBS lasI - ecfp
D107 (BV) - D131 (BI)

To do again
L124

Strongest RBS - rfp
D102 (BV) - D122 (BI)

To do again
L125

Strongest RBS - yfp
D102 (BV) - D118 (BI)

To do again
L126

Strongest RBS (1)- LacR activator (+LVA)
D102 (BV) - D118 (BI)

To do again
L127

gfp (1)- Double terminator
D119 (FI) - D125 (FV)

To do again
Controls
C1

D110

-
C2

D129

-
C3

D123

-
C4

D103

-
C5

D124

-
C6

D104

-
C7

D126

-
C8

D105

-
C9

D125

-
C10

D106

-
C11

D107

-
C12

D102

-
Positive control

puc19

-
Retrieved from "http://2008.igem.org/Team:Paris/July_31"