"
Team:Paris/July 31
From 2008.igem.org
(→Protocol) |
(→Ligations) |
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== Ligations == | == Ligations == | ||
| - | We tried again the following ligations : | + | ''We tried again the following ligations : |
| - | L100, L101, L109, L113, L114, L120, L122, L123, L126, L127 | + | L100, L101, L109, L113, L114, L120, L122, L123, L126, L127'' |
=== Protocol === | === Protocol === | ||
| - | For each samples, | + | ''For each samples,'' |
* 1 µl Ligase | * 1 µl Ligase | ||
| Line 162: | Line 162: | ||
* 2 µl Ligase Buffer 10x | * 2 µl Ligase Buffer 10x | ||
* 20 µl qsp H2O | * 20 µl qsp H2O | ||
| - | |||
* Incubates O/N at 4°C (in the fridge) | * Incubates O/N at 4°C (in the fridge) | ||
| + | === List of new Ligation transformation === | ||
{| border="1" | {| border="1" | ||
Revision as of 13:00, 5 August 2008
|
Results of the transformation : Fluorescence
LigationsWe tried again the following ligations : L100, L101, L109, L113, L114, L120, L122, L123, L126, L127 ProtocolFor each samples,
List of new Ligation transformation
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