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Team:Paris/August 8
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{{Paris/Calendar_Links|August 7|August 9}} | {{Paris/Calendar_Links|August 7|August 9}} | ||
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| + | == Minipreps : Plasmid extraction== | ||
| + | |||
| + | Extraction of '''pSB3K3 et E0240 in pSB1A2 plasmid''' from overnight bacteria culture using the QIAspin Miniprep Kit (QIAGEN) by QIACube. | ||
| + | |||
| + | * Carried out 2 times (2 tubes) | ||
| + | |||
| + | *{| border="1" | ||
| + | |'''name''' | ||
| + | |'''Biobrick''' | ||
| + | |'''plasmid''' | ||
| + | |- | ||
| + | |align="center"|MP142 | ||
| + | | - | ||
| + | |align="center"|pSB3K3 | ||
| + | |- | ||
| + | |align="center"|MP143 | ||
| + | |align="center"|E0240 | ||
| + | |align="center"|pSB1A2 | ||
| + | |} | ||
| + | <br> | ||
| + | |||
==Transformation results== | ==Transformation results== | ||
Revision as of 09:11, 11 August 2008
|
Minipreps : Plasmid extractionExtraction of pSB3K3 et E0240 in pSB1A2 plasmid from overnight bacteria culture using the QIAspin Miniprep Kit (QIAGEN) by QIACube.
| name | Biobrick | plasmid |
| MP142 | - | pSB3K3 | |
| MP143 | E0240 | pSB1A2 |
Transformation results
| Name | Description | Antibio | Number of colonies | Number of red fluorescent colonies |
| Ligation | ||||
| L128 | J61002-pFlgA D136 (FV) - D132 (FI) | Amp | ~ 400 | 2 |
| L129 | J61002-pFlgB D136 (FV) - D133 (FI) | Amp | 39 | 5 |
| L130 | J61002-pFlhB D136 (FV) - D134 (FI) | Amp | ~ 1000 | 4 (but 3 are on the edge of the petri dishe) |
| L131 | J61002-pFlhDC D136 (FV) - D135 (FI) | Amp | 39 | 38 |
| Control | ||||
| Control 1 | D136 | Amp | 0 | 0 |
| Positive control | pUC19 | Amp | 36 | 0 |
