Team:Paris/August 11

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===Culture of ligation transformants===
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*4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for PCR screening).
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*37°C overnight

Revision as of 16:03, 11 August 2008

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Culture of ligation transformants

  • 4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for PCR screening).
  • 37°C overnight
Retrieved from "http://2008.igem.org/Team:Paris/August_11"