Team:Paris/August 11

From 2008.igem.org

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(→Culture of ligation transformants)
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*4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for PCR screening).
*4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for PCR screening).
*37°C overnight
*37°C overnight
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 +
{| Border="1"
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|align="center"|'''Ligation'''
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|align="center"|'''Name'''
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|align="center"|'''Clone N°'''
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|align="center"|'''Red Fluorescence'''
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|-
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|align="center"|L128
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|align="center"|pFlgA
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|align="center"|
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|align="center"|
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|-
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|align="center"|L129
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|align="center"|pFlgB
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|align="center"|
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|align="center"|
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|-
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|align="center"|L130
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|align="center"|pFlhB
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|align="center"|
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|align="center"|
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|-
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|align="center"|L131
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|align="center"|pFlhDC
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|align="center"|
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|align="center"|
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|-
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|}

Revision as of 16:06, 11 August 2008

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Culture of ligation transformants

  • 4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for PCR screening).
  • 37°C overnight
Ligation Name Clone N° Red Fluorescence
L128 pFlgA
L129 pFlgB
L130 pFlhB
L131 pFlhDC
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