Team:Paris/August 11

From 2008.igem.org

(Difference between revisions)
(→Culture of ligation transformants)
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{|| Border="1"
{|| Border="1"
|align="center"|'''Ligation'''
|align="center"|'''Ligation'''
-
| colspan="8" align="center"|L128
+
| colspan="4" align="center"|L128
-
| colspan="8" align="center"|L129
+
| colspan="4" align="center"|L129
-
| colspan="8" align="center"|L130
+
| colspan="4" align="center"|L130
|-
|-
|align="center"|'''Name'''
|align="center"|'''Name'''
-
| colspan="8" align="center"|pFlgA
+
| colspan="4" align="center"|pFlgA
-
| colspan="8" align="center"|pFlgB
+
| colspan="4" align="center"|pFlgB
-
| colspan="8" align="center"|pFlhB
+
| colspan="4" align="center"|pFlhB
|-
|-
|align="center"|''Clone N°'''
|align="center"|''Clone N°'''

Revision as of 16:14, 11 August 2008

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Culture of ligation transformants

  • 4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for PCR screening).
  • 37°C overnight
Ligation Name Clone N° Red Fluorescence
L128 pFlgA
L129 pFlgB
L130 pFlhB
L131 pFlhDC
Ligation L128 L129 L130
Name pFlgA pFlgB pFlhB
Clone N°' 1 2 3 4 1 2 6 7 1 2 7 8
Red fluorescence yes yes no no yes yes no no yes no no no
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