Team:Paris/August 11

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(→Culture of ligation transformants)
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| colspan="4" align="center"|pFlhB
| colspan="4" align="center"|pFlhB
|-
|-
-
|align="center"|''Clone N°'''
+
|align="center"|'''Clone N°'''
|align="center"|1
|align="center"|1
|align="center"|2
|align="center"|2

Revision as of 16:14, 11 August 2008

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Culture of ligation transformants

  • 4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for PCR screening).
  • 37°C overnight
Ligation Name Clone N° Red Fluorescence
L128 pFlgA
L129 pFlgB
L130 pFlhB
L131 pFlhDC
Ligation L128 L129 L130
Name pFlgA pFlgB pFlhB
Clone N° 1 2 3 4 1 2 6 7 1 2 7 8
Red fluorescence yes yes no no yes yes no no yes no no no
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