Team:Paris/August 11

From 2008.igem.org

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(→Culture of ligation transformants)
(→Culture of ligation transformants)
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*4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for PCR screening).
*4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for PCR screening).
*37°C overnight
*37°C overnight
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{| Border="1"
 
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|align="center"|'''Ligation'''
 
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|align="center"|'''Name'''
 
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|align="center"|'''Clone N°'''
 
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|align="center"|'''Red Fluorescence'''
 
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|-
 
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|align="center"|L128
 
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|align="center"|pFlgA
 
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|align="center"|
 
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|align="center"|
 
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|-
 
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|align="center"|L129
 
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|align="center"|pFlgB
 
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|align="center"|
 
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|align="center"|
 
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|-
 
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|align="center"|L130
 
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|align="center"|pFlhB
 
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|align="center"|
 
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|align="center"|
 
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|-
 
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|align="center"|L131
 
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|align="center"|pFlhDC
 
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|align="center"|
 
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|align="center"|
 
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|-
 
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|}
 
{|| Border="1"
{|| Border="1"

Revision as of 16:14, 11 August 2008

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Culture of ligation transformants

  • 4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for PCR screening).
  • 37°C overnight
Ligation L128 L129 L130
Name pFlgA pFlgB pFlhB
Clone N° 1 2 3 4 1 2 6 7 1 2 7 8
Red fluorescence yes yes no no yes yes no no yes no no no
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