Team:Paris/August 11

(Difference between revisions)

Revision as of 16:18, 11 August 2008

4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for the PCR screening of August 8).
37°C overnight

Ligation

L128

L129

L130

Name

pFlgA

pFlgB

pFlhB

Clone N°

1

2

3

4

1

2

6

7

1

2

7

8

Red fluorescence

yes

no

yes

no

yes

no

@@ Line 3: / Line 3: @@
 ===Culture of ligation transformants===
-*4 clones of each transformation were cultured in '''7,5 mL LB + ampicilline'''. The colonies picked up were the rest of those already picked up from the transformation plate (for PCR screening).
+*4 clones of each transformation were cultured in '''7,5 mL LB + ampicilline'''. The colonies picked up were the rest of those already picked up from the transformation plate (for the PCR screening of August 8).
 *37°C overnight