Team:Paris/August 11

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{{Paris/Calendar_Links|August 10|August 12}}
{{Paris/Calendar_Links|August 10|August 12}}
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===Culture of ligation transformants===
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==Culture of ligation transformants==
*4 clones of each transformation were cultured in '''7,5 mL LB + ampicilline'''. The colonies picked up were the rest of those already picked up from the transformation plate (for the PCR screening of August 8).
*4 clones of each transformation were cultured in '''7,5 mL LB + ampicilline'''. The colonies picked up were the rest of those already picked up from the transformation plate (for the PCR screening of August 8).

Revision as of 16:18, 11 August 2008

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Culture of ligation transformants

  • 4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for the PCR screening of August 8).
  • 37°C overnight
Ligation L128 L129 L130
Name pFlgA pFlgB pFlhB
Clone N° 1 2 3 4 1 2 6 7 1 2 7 8
Red fluorescence yes yes no no yes yes no no yes no no no
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