Team:Paris/August 11

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==Transformation==
==Transformation==
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* [https://2008.igem.org/Team:Paris/Notebook/Protocols Protocol] : (see # '''13''')
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* [https://2008.igem.org/Team:Paris/Notebook/Protocols Protocol for Top 10 cells] : (see # '''13''')
==Digestion==
==Digestion==

Revision as of 18:09, 11 August 2008

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Contents

Transformation

Digestion

PCR

Culture of ligation transformants

  • 4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for the PCR screening of August 8).
  • 37°C overnight
Ligation L128 L129 L130
Name pFlgA pFlgB pFlhB
Clone N° 1 2 3 4 1 2 6 7 1 2 7 8
Red fluorescence yes yes no no yes yes no no yes no no no
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