Team:Paris/August 13

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(New page: {{Paris/Calendar_Links|August 12|August 14}} == Minipreps: Plasmid extraction== * [https://2008.igem.org/Team:Paris/Notebook/Protocols#Minipreps_.28Kit_Qiagen.29 Protocol] (see # '''3'''...)
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Revision as of 14:55, 13 August 2008

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Minipreps: Plasmid extraction

Protocol (see # 3) Experiments done by QIAcube

List of Minipreps

Name	Ligation	Biobricks	Description
MP147.1	L100.1	rbs TetR - ECFP D110 (BV) - D130 (BI)
MP147.2	L100.2
MP147.3	L100.3
MP148.1	L101.1	rbs TetR - GFP tripart D110 (BV) - D131 (BI)
MP148.2	L101.2
MP148.3	L101.3
MP149.1	L114.1	AracpBAD - gfp tripart D126 (BV) - D131 (BI)
MP149.2	L114.2
MP149.3	L114.3
MP150.1	L120.1	tetR repressible promoter - ECFP D106 (BV) - D130 (BI)
MP150.2	L120.2
MP150.3	L120.3
MP151.1	L122.1	RBS-lasI - ecfp D107 (BV) - D130 (BI)
MP152.1	L123.1	RBS lasI - ecfp D107 (BV) - D131 (BI)
MP151.2	L123.2
MP152.3	L123.3
MP153.1	L126.1	Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)
MP153.2	L126.2
MP153.3	L126.3
MP154.1	L132.1
MP154.2	L132.2
MP154.3	L132.3
MP155.1	L133.1
MP156.1	L134.1
MP156.2	L134.2
MP156.3	L134.3
MP157.1	L138.1

Glycerol Stocks

Protocol (see #2)

List of Stocks

Strain	Ligation	Biobricks	Description
S143.1	L128.1	pFlgA-RFP D132 (FI) - D136 (FV)
S143.2	L128.2
S143.3	L128.3
S143.4	L128.4
S144.1	L129.1	pFlgB-RFP D133 (FI) - D136 (FV)
S144.2	L129.2
S144.6	L129.6
S144.7	L129.7
S145.1	L130.1	pFlhB-RFP D134 (FI) - D136 (FV)
S145.2	L130.2
S145.7	L130.7
S145.8	L130.8