Team:Paris/August 15

From 2008.igem.org

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(Analysis of yesterday PCR screening)
(Analysis of yesterday PCR screening)
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==Analysis of yesterday PCR screening==
==Analysis of yesterday PCR screening==
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Revision as of 15:08, 15 August 2008

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Contents

Transformation of the ligations we did yesterday

We transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells
standard protocol.


Digestion

Measurement of concentration of minipreps

standard protocol

Digestion Miniprep used Concentration (µg/mL) ratio 260/280
D146 MP148.2 123 1.58
D147 MP153.3 113 1.68


Digestion

Protocol Digestion


Electrophoresis for extraction

standard protocol

Analysis of yesterday PCR screening

KR000163.jpg KR000162.jpg