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Team:Paris/August 15
From 2008.igem.org
(Difference between revisions)
AnaJimenez (Talk | contribs) (→Starting the construction of the Promoter characterization plasmid) |
AnaJimenez (Talk | contribs) (→Measurement of concentration of minipreps) |
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==Digestion== | ==Digestion== | ||
| - | =Measurement of concentration of minipreps= | + | ===Measurement of concentration of minipreps=== |
[[Team:Paris/Notebook/Protocols#Concentration of the Miniprep|standard protocol]] | [[Team:Paris/Notebook/Protocols#Concentration of the Miniprep|standard protocol]] | ||
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|} | |} | ||
| - | ==Digestion== | + | ===Digestion=== |
[[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]] | [[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]] | ||
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|- | |- | ||
|MP3 | |MP3 | ||
| - | | | + | |B0015 (double terminator B0010-B0012) |
| - | | | + | |4 |
| - | | | + | |EcoRI and XbaI |
|- | |- | ||
| - | | | + | |MP114 |
| - | | | + | |TetR |
| - | | | + | |1 |
| - | |1 | + | |EcoRI and SpeI |
| + | |- | ||
| + | |MP104 | ||
| + | |PTet (Tet promoter) | ||
| + | |1 | ||
| + | |SpeI and PstI | ||
| + | |- | ||
| + | |MP101 | ||
| + | |promoter J23101 | ||
| + | |1 | ||
| + | |SpeI and PstI | ||
| + | |- | ||
| + | |MP143 | ||
| + | |gfp generator | ||
| + | |2 | ||
| + | |SpeI and PstI | ||
|} | |} | ||
Revision as of 16:15, 15 August 2008
Transformation of the ligations we did yesterdayWe transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells
DigestionMeasurement of concentration of minipreps
Digestion
Starting the construction of the Promoter characterization plasmidDigestionMeasurement of concentration of minipreps
Digestion
Analysis of yesterday PCR screening
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