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Team:Paris/August 15
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=='''Transformation of the ligations we did [[Team:Paris/August 14| yesterday]]'''== | =='''Transformation of the ligations we did [[Team:Paris/August 14| yesterday]]'''== | ||
We transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells [[Team:Paris/Notebook/Protocols#Transformation|standard protocol]]. | We transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells [[Team:Paris/Notebook/Protocols#Transformation|standard protocol]]. | ||
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| + | =='''PCR amplification of flhDC and its promoter'''== | ||
| + | ==='''List of PCRs'''=== | ||
| + | ==='''Protocol'''=== | ||
| + | ==='''Results'''=== | ||
| + | ==='''Digestions'''=== | ||
=='''Digestion'''== | =='''Digestion'''== | ||
Revision as of 14:28, 16 August 2008
Transformation of the ligations we did yesterdayWe transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells standard protocol. PCR amplification of flhDC and its promoterList of PCRsProtocolResultsDigestionsDigestionMeasurement of concentration of minipreps
DigestionLigation
Analysis of yesterday PCR screening
Starting the construction of the Promoter characterization plasmidDigestionMeasurement of concentration of minipreps
Digestion
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