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Team:Paris/August 15
From 2008.igem.org
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=='''PCR amplification of flhDC and its promoter'''== | =='''PCR amplification of flhDC and its promoter'''== | ||
| + | [[Image:KR000170.jpg|thumb|Gel 1%]] | ||
| + | [[Image:KR000171.jpg|thumb|Gel 2%]] | ||
==='''List of PCRs'''=== | ==='''List of PCRs'''=== | ||
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Revision as of 15:10, 16 August 2008
Transformation of the ligations we did yesterdayWe transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells standard protocol. PCR amplification of flhDC and its promoter  List of PCRs
ProtocolWe followed the standard protocol of amplification in Two steps. PCR program used : PHUSION2 Results DigestionsDigestionMeasurement of concentration of minipreps
DigestionLigation
Analysis of yesterday PCR screeningGel 1
Starting the construction of the Promoter characterization plasmidDigestionMeasurement of concentration of minipreps
Digestion
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