Team:Paris/August 18

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(New page: {{Paris/Calendar_Links|August 17|August 19}} =Analysis of the cloning of OmpR and EnvZ= ==PCR screening== *12,5 microL Quick load PCR Mix *0,5 microL O18 *0,5 microL O19 *12 microL wate...)
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Revision as of 18:16, 18 August 2008

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Analysis of the cloning of OmpR and EnvZ

PCR screening

  • 12,5 microL Quick load PCR Mix
  • 0,5 microL O18
  • 0,5 microL O19
  • 12 microL water



  • positive control: S158 (pSB3K3)
  • negative control: no template
  • screening PCR programm
  • elongation time: 1 min 30
  • primers: O18 & O19


We wanted to analyse the transformants of the ligations L133 (OmpR in pSB1A2) and L134 (EnvZ in pSB1A2) from the experience of august 3 clones of L133 and 3 clones of L134 were analysed.
Each clone tested was then cultured in 7,5 mL LB + ampicilline.

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