Team:Paris/August 20

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{{Paris/Calendar_Links|August 19|August 21}}
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=Screening of the cloning of E0240 and FlhDC+promotor=
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==Spreading the clones in  order to obtain single colonies==
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{|border="1" style="text-align: center"
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|'''Strain'''
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|'''Resistance'''
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|'''Ligation'''
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|'''DNA cloned'''
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|'''vector'''
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|'''expected size of the fragment amplified by VF & VR'''
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|'''mesured size'''
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|-
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|S159.1
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|kanamycine
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|L139.1
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|E0240 (GFP tripart)
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|pSB3K3
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|1192 bp
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|1,5 kb<br>'''1,1 kb'''<br>0,6 kb
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|-
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|S161.1
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|ampicilline
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|L142.7
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|FlhDC+promotor
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|pSB1A2
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|1403 bp
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|'''1,4'''<br>0,4 kb<br>0,3 kb
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|}
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The plates obtained from the speading of yesterday can't be used because there are not single colonies.
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<br>We have to try again, but with a stronger dilution of the bacteria or with a smaller volume of spreading.

Revision as of 09:55, 20 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Screening of the cloning of E0240 and FlhDC+promotor

Spreading the clones in order to obtain single colonies

Strain Resistance Ligation DNA cloned vector expected size of the fragment amplified by VF & VR mesured size
S159.1 kanamycine L139.1 E0240 (GFP tripart) pSB3K3 1192 bp 1,5 kb
1,1 kb
0,6 kb
S161.1 ampicilline L142.7 FlhDC+promotor pSB1A2 1403 bp 1,4
0,4 kb
0,3 kb

The plates obtained from the speading of yesterday can't be used because there are not single colonies.
We have to try again, but with a stronger dilution of the bacteria or with a smaller volume of spreading.

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