Team:Paris/August 20
From 2008.igem.org
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Revision as of 18:07, 20 August 2008
Screening of the cloning of E0240 and FlhDC+promotorSpreading the clones in order to obtain single colonies
The plates obtained from the speading of yesterday can't be used because there are not single colonies.
Miniprep and stock glycerolStock Glycerol of New BiobrickMiniprep of New BiobricksDigestion of MP162Measurement of the concentration of MPs 162
Digestion
Gel Extraction
Ligation
Culture of stable strains with biobrocks 2008
Construction of pFlgA - YFP tripartAim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionMeasurement of concentration of minipreps
Digestion
Gel Extraction
Results of the transformation we did yesterdayNumber of colonies
PCR Screening==> Protocol [[Image:]]
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