Team:Paris/August 20
From 2008.igem.org
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==='''Gel Extraction'''=== | ==='''Gel Extraction'''=== | ||
- | [[Image: | + | [[Image:KR000193b.jpg| thumb|Gel Extraction of D166-D167-D131]] |
[[Team:Paris/Notebook/Protocols#Migration after extraction |Protocol]] | [[Team:Paris/Notebook/Protocols#Migration after extraction |Protocol]] | ||
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|MP165.1 | |MP165.1 | ||
|2 957 pb | |2 957 pb | ||
- | |style="background: #cbff7B"| | + | |style="background: #cbff7B"| 3 000 pb |
|- | |- | ||
|3 | |3 | ||
|MP166.1 | |MP166.1 | ||
|2 996 pb | |2 996 pb | ||
- | |style="background: #cbff7B"| | + | |style="background: #cbff7B"| 3 000 pb |
|- | |- | ||
|4 | |4 | ||
|MP122.2 | |MP122.2 | ||
|2 957 pb | |2 957 pb | ||
- | |style="background: #cbff7B"| | + | |style="background: #cbff7B"| 3 000 pb |
|- | |- | ||
|5 | |5 | ||
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|D166 | |D166 | ||
|885 pb | |885 pb | ||
- | |style="background: # | + | |style="background: #ff6d73"| 2 500 pb |
|- | |- | ||
|7 | |7 | ||
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|D167 | |D167 | ||
|914 pb | |914 pb | ||
- | |style="background: # | + | |style="background: #ff6d73"| 2 500 pb |
|- | |- | ||
|9 | |9 | ||
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|D131 | |D131 | ||
|900 pb | |900 pb | ||
- | |style="background: #cbff7B"| | + | |style="background: #cbff7B"| 950 pb |
|- | |- | ||
|11 | |11 |
Revision as of 09:49, 21 August 2008
Screening of the cloning of E0240 and FlhDC+promotorSpreading the clones in order to obtain single colonies
The plates obtained from the speading of yesterday can't be used because there are not single colonies.
Miniprep and stock glycerolStock Glycerol of New BiobrickMiniprep of New Biobricks
Construction of pFlgA - YFP tripart (+/- LVA)Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionMeasurement of concentration of minipreps
Digestion
Gel Extraction
Results of the transformation we did yesterdayNumber of colonies
PCR Screening==> Protocol [[Image:]]
Minipreps
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