Team:Paris/August 22

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(Difference between revisions)
(→Transformation of the ligations we did yesterday)
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='''Transformation of the ligations we did [[Team:Paris/August 21 |yesterday]]'''=
='''Transformation of the ligations we did [[Team:Paris/August 21 |yesterday]]'''=
 +
[[Team:Paris/Notebook/Protocols#Transformation |Protocol]]
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{|border="1" style="text-align:center"
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|'''Ligation Name'''
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|'''Description'''
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|'''Antibio'''
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|-
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|L158
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|D110(BV) + D131 (BI)
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|Amp
 +
|-
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|T L158
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|D110
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|Amp
 +
|-
 +
|L159
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|D125(FV)+D109(FI)
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|Kan
 +
|-
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|T L159
 +
|D125
 +
|Kan
 +
|}
='''Construction for synchronization'''=
='''Construction for synchronization'''=

Revision as of 15:21, 22 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Contents

Analysis of the transformant of FlhDC+promotor

Transformation of the ligations we did yesterday

Protocol

Ligation Name Description Antibio
L158 D110(BV) + D131 (BI) Amp
T L158 D110 Amp
L159 D125(FV)+D109(FI) Kan
T L159 D125 Kan

Construction for synchronization

Ligations

Protocol

Ligation Name Description Vector Name Volume vector (µL) Insert Name Volume insert(µL)
L162 rbs-lasI + gfp generator
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png 		D107 (BV) 10 D163 (BI) 3.46
T L162 autoligation control D107 (BV) 10
L163 rbs-TetR + gfp generator
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png 		D110 (BV) 2.5 D163 (BI) 3.44
T L163 autoligation control D110 (BV) 2.5
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