Team:Paris/August 24

From 2008.igem.org

(Difference between revisions)
(→Construction for synchronization)
(→Ligation)
Line 125: Line 125:
=='''Ligation'''==
=='''Ligation'''==
 +
[[Team:Paris/Notebook/Protocols#Ligation |Protocol]]
 +
 +
{|border="1" style="text-align: center"
 +
|'''Ligation Name'''
 +
|'''Vector Name'''
 +
|'''Volume Vector (µL)'''
 +
|'''Insert'''
 +
|'''Volume Insert (µL)'''
 +
|-
 +
|L160
 +
|D166
 +
|3.63
 +
|D132
 +
|2.03
 +
|-
 +
|L161
 +
|D167
 +
|4.00
 +
|D132
 +
|2.01
 +
|-
 +
|Control L160
 +
|D166
 +
|3.63
 +
| -
 +
| -
 +
|-
 +
|Control L161
 +
|D167
 +
|4.00
 +
| -
 +
| -
 +
|}

Revision as of 12:52, 25 August 2008

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Construction for synchronization

Gel extraction

Protocol

Gel 1 (no image sorry, we forgot to save it!)

Well 1 2 3 4 5 6 7 8 9 10 11 12
Sample 1kb ladder MP105.1 no sample D107 no sample D109 no sample MP106 no sample D110 no sample 100pb ladder
Expected size (pb) - 2764 - 2750
14 		- 2056
708 		- 2782 - 2768
14 		- -
Measured size (pb) - 2800 - 2750 - 2000
700 		- 2800 - 2750 - -

Gel 2

Well 1 2 3 4 5 6 7 8 9 10 11 12
Sample no sample 1kb ladder MP118.1 no sample D125 no sample MP143 no sample D163 no sample 100pb ladder no sample
Expected size (pb) - - 3318 - 3303
15 		- 3322 - 2432
890 		- - -
Measured size (pb) - - 2900 - 3300 - 2900 - 2500
900 		- - -

Ligation

Protocol

Ligation Name Vector Name Volume Vector (µL) Insert Volume Insert (µL)
L160 D166 3.63 D132 2.03
L161 D167 4.00 D132 2.01
Control L160 D166 3.63 - -
Control L161 D167 4.00 - -
Retrieved from "http://2008.igem.org/Team:Paris/August_24"