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Team:Paris/August 25
From 2008.igem.org
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==Electrophoresis== | ==Electrophoresis== | ||
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| + | [[Image:KR000225.jpg|thumb|]] | ||
| + | [[Image:KR000226.jpg|thumb|Gel after excision]] | ||
1% agarose gel | 1% agarose gel | ||
*EnvZ*: 3 µL of digestion products + 1 µL of loading blue + 2 µL of water | *EnvZ*: 3 µL of digestion products + 1 µL of loading blue + 2 µL of water | ||
*pSB1A2: 30 µL of digestion products + 6 µL of loading blue | *pSB1A2: 30 µL of digestion products + 6 µL of loading blue | ||
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{|border="1" style="text-align: center" | {|border="1" style="text-align: center" | ||
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|1 kb DNA ladder | |1 kb DNA ladder | ||
|D159 (EnvZ*) | |D159 (EnvZ*) | ||
| - | | | + | |nothing |
|D116 (pSB1A2 & lasR) | |D116 (pSB1A2 & lasR) | ||
| - | | | + | |nothing |
|100 bp DNA ladder | |100 bp DNA ladder | ||
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Revision as of 10:00, 26 August 2008
Construction for SynchronizationTransformation of the ligations we did yesterday
Construction of pFlgA - GFP GeneratorAim : Construction of "pFlgA-RBS-GFP-dbl ter" (pFlgA-E0240)
Results of the transformation we did the day before yesterday
=> Need to screen to know which clones we can use for the of pFlgA promotor characterization. Cloning of EnvZ*The sequencing of EnvZ* previously cloned, revealed a loss of about 300 bp. EnvZ* contains indeed an EcoRI restriction site within its sequence. So we can't use this enzyme during the cloning. Digestion
Reaction mixture
Incubation at 37°C during 2H25, and then ~20 min at 65°C Electrophoresis  1% agarose gel
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