Team:Paris/August 26
From 2008.igem.org
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{{Paris/Calendar_Links|August 25|August 27}} | {{Paris/Calendar_Links|August 25|August 27}} | ||
+ | |||
+ | =PCR= | ||
+ | |||
+ | * pFlhDC (O111-F / O113-R) | ||
+ | * Gene FlhDC (O134-F / O131-R) | ||
+ | * pFlgB (O102-F / O103-R) | ||
+ | * pFlhB (O108-F / O109-R) | ||
+ | -- | ||
+ | *Program: Gradient | ||
+ | Denaturation : 98°C-5'<br> | ||
+ | Cycling 1 (3X) : 98°C-10''<br> | ||
+ | Gradient 61°C +/-10°C - 30''<br> | ||
+ | 72°C-30''<br> | ||
+ | Cycling 2 (25X) : 98°C-10''<br> | ||
+ | 72°C-30''<br> | ||
+ | Elongation : 72°C-5'<br> | ||
+ | -- | ||
+ | Vf=20µL<br> | ||
+ | H20=13,4µL<br> | ||
+ | Buffer5X=4µL<br> | ||
+ | dNTP=0,4µL<br> | ||
+ | O1=1µL<br> | ||
+ | O2=1µL<br> | ||
+ | Phusion=0,2µL<br> | ||
=Cloning of EnvZ*= | =Cloning of EnvZ*= |
Revision as of 10:20, 26 August 2008
PCR
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Denaturation : 98°C-5' Gradient 61°C +/-10°C - 30 Cycling 2 (25X) : 98°C-10 72°C-30 Elongation : 72°C-5' Cloning of EnvZ*Concentration measurement by Biophotometer
Ligation |