Team:Paris/August 26

From 2008.igem.org

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{{Paris/Calendar_Links|August 25|August 27}}
{{Paris/Calendar_Links|August 25|August 27}}
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=PCR=
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='''Bold text'''PCR=
* pFlhDC (O111-F / O113-R)
* pFlhDC (O111-F / O113-R)
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*Program: Gradient
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'''Program: Gradient'''
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Denaturation : 98°C-5'<br>
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'''''Denaturation :'''''
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Cycling 1 (3X) : 98°C-10''
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98°C-5'
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&nbsp Gradient 61°C +/-10°C - 30''
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'''''Cycling 1 (3X) :'''''
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                            72°C-30''
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98°C-10''
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Cycling 2 (25X) : 98°C-10''
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Gradient 61°C +/-10°C - 30''
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                              72°C-30''
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72°C-30''
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Elongation : 72°C-5'
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'''''Cycling 2 (25X) :'''''
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98°C-10''
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72°C-30''
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'''''Elongation :'''''
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72°C-5'
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Revision as of 10:24, 26 August 2008

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Contents

Bold textPCR

  • pFlhDC (O111-F / O113-R)
  • Gene FlhDC (O134-F / O131-R)
  • pFlgB (O102-F / O103-R)
  • pFlhB (O108-F / O109-R)

Program: Gradient Denaturation : 98°C-5' Cycling 1 (3X) : 98°C-10 Gradient 61°C +/-10°C - 30 72°C-30 Cycling 2 (25X) : 98°C-10 72°C-30 Elongation : 72°C-5'

Vf=20µL
H20=13,4µL
Buffer5X=4µL
dNTP=0,4µL
O1=1µL
O2=1µL
Phusion=0,2µL

Cloning of EnvZ*

Concentration measurement by Biophotometer

Name [DNA] in µg/mL A260/A280
EnvZ* 10 µg/mL 1.35
pSB1A2 17 µg/mL 1.02

Ligation
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