Team:Paris/August 28
From 2008.igem.org
(Difference between revisions)
(→Cloning of EnvZ*) |
(→Cloning of EnvZ*) |
||
Line 45: | Line 45: | ||
=Cloning of EnvZ*= | =Cloning of EnvZ*= | ||
+ | |||
+ | The cloning of EnvZ* didn't worked. So we decided to try one more time, starting from the PCR product. | ||
==Digestion== | ==Digestion== | ||
Line 67: | Line 69: | ||
|XbaI & PstI | |XbaI & PstI | ||
|} | |} | ||
+ | <br> | ||
+ | '''Purification''' | ||
+ | *EnvZ*: directly by the Qiagen kit | ||
+ | *pSB1A2: excision of the band of interest (2 kb) and then purification using the Qiagen kit |
Revision as of 12:39, 29 August 2008
Transformation of OmpR*Construction of pFlhB - mRFP Tripart (LVA+)Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth. DigestionMeasurement of the concentration of D187 purifiedProtocol (it's same that for Miniprep)
Ligation
Cloning of EnvZ*The cloning of EnvZ* didn't worked. So we decided to try one more time, starting from the PCR product. Digestion
|