Team:Paris/September 2

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(Difference between revisions)
(→Ligation L173 (rbs-TetR in mRFP_LVA_tripart-pSB1A3))
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{{Paris/Calendar_Links|September 1|September 3}}
{{Paris/Calendar_Links|September 1|September 3}}
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=Ligation L173 (rbs-TetR in mRFP_LVA_tripart-pSB1A3)=
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=Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3)=
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{|border="1" style="text-align: center"
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|'''Name'''
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|'''Digestion number'''
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|'''Digested by'''
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|-
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|rbs-TetR
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|D112
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|EcoRI & SpeI (insert)
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|-
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|mRFP-LVA-tripart
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|D187
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|EcoRI & XbaI (vector)
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|-
 +
|}
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{|border="1" style="text-align: center"
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|rowspan="2"|
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|rowspan="2"|Control
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|colspan="2"|Sample
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|-
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|'''3:1''' (insert:vector) '''ratio'''
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|'''4:1''' (insert:vector) '''ratio'''
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|-
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|D112
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|0 µL
 +
|1,4 µL
 +
|1,9 µL
 +
|-
 +
|D187
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|2,5 µL
 +
|2,5 µL
 +
|2,5 µL
 +
|-
 +
|T4 DNA ligase 10X buffer
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|2 µL
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|2 µL
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|2 µL
 +
|-
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|T4 DNA ligase
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|1 µL
 +
|1 µL
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|1 µL
 +
|-
 +
|water
 +
|14,5 µL
 +
|13,1 µL
 +
|12,6 µL
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|-
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|}

Revision as of 16:21, 2 September 2008

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Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3)

Name Digestion number Digested by
rbs-TetR D112 EcoRI & SpeI (insert)
mRFP-LVA-tripart D187 EcoRI & XbaI (vector)
Control Sample
3:1 (insert:vector) ratio 4:1 (insert:vector) ratio
D112 0 µL 1,4 µL 1,9 µL
D187 2,5 µL 2,5 µL 2,5 µL
T4 DNA ligase 10X buffer 2 µL 2 µL 2 µL
T4 DNA ligase 1 µL 1 µL 1 µL
water 14,5 µL 13,1 µL 12,6 µL
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