Team:Paris/September 2

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(Difference between revisions)
(→Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3))
(→Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3))
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|'''Digestion number'''
|'''Digestion number'''
|'''Digested by'''
|'''Digested by'''
 +
|'''DNA concentration'''
|-
|-
|rbs-TetR
|rbs-TetR
|D112
|D112
|EcoRI & SpeI (insert)
|EcoRI & SpeI (insert)
 +
|~20 µg/mL
|-
|-
|mRFP-LVA-tripart
|mRFP-LVA-tripart
|D187
|D187
|EcoRI & XbaI (vector)
|EcoRI & XbaI (vector)
 +
|16 µg/mL
|-
|-
|}
|}

Revision as of 16:28, 2 September 2008

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Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3)

Name Digestion number Digested by DNA concentration
rbs-TetR D112 EcoRI & SpeI (insert) ~20 µg/mL
mRFP-LVA-tripart D187 EcoRI & XbaI (vector) 16 µg/mL


Control Sample
3:1 (insert:vector) ratio 4:1 (insert:vector) ratio
D112 (insert) 0 µL 1,4 µL 1,9 µL
D187 (vector) 2,5 µL 2,5 µL 2,5 µL
T4 DNA ligase 10X buffer 2 µL 2 µL 2 µL
T4 DNA ligase 1 µL 1 µL 1 µL
water 14,5 µL 13,1 µL 12,6 µL
  • Put on the room "Danger azote" at about 16°C at 18h00.
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