Team:Paris/September 2

From 2008.igem.org

(Difference between revisions)
(→Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3))
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|12,6 µL
|12,6 µL
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*Put in the room "Danger azote" at about 16°C at 18h00 (for overnight reaction).
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<br>
=Ligation L172 ('''rbs-FlhDC''' in '''mRFP-LVA-tripart'''-pSB1A3)=
=Ligation L172 ('''rbs-FlhDC''' in '''mRFP-LVA-tripart'''-pSB1A3)=
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|2,5 µL
|2,5 µL
|2,5 µL
|2,5 µL
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|T4 DNA ligase 10X '''buffer'''
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|2 µL
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|2 µL
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|T4 DNA '''ligase'''
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|1 µL
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|1 µL
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|'''water'''
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|14,5 µL
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|13,5 µL
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*Put in the room "Danger azote" at about 16°C at 18h00 (for overnight reaction).
*Put in the room "Danger azote" at about 16°C at 18h00 (for overnight reaction).

Revision as of 17:07, 2 September 2008

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Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3)

Samples used

Name Digestion number Digested by DNA concentration
rbs-TetR D112 EcoRI & SpeI (insert) ~20 µg/mL
mRFP-LVA-tripart D187 EcoRI & XbaI (vector) 16 µg/mL


Reaction mixtures

Control Sample
3:1 (insert:vector) ratio 4:1 (insert:vector) ratio
D112 (insert) 0 µL 1,4 µL 1,9 µL
D187 (vector) 2,5 µL 2,5 µL 2,5 µL
T4 DNA ligase 10X buffer 2 µL 2 µL 2 µL
T4 DNA ligase 1 µL 1 µL 1 µL
water 14,5 µL 13,1 µL 12,6 µL
  • Put in the room "Danger azote" at about 16°C at 18h00 (for overnight reaction).


Ligation L172 (rbs-FlhDC in mRFP-LVA-tripart-pSB1A3)

Samples used

Name Digestion number Digested by DNA concentration
rbs-FlhDC D186 EcoRI & SpeI (insert) ~24 µg/mL
mRFP-LVA-tripart D187 EcoRI & XbaI (vector) 16 µg/mL


Reaction mixtures

Control Sample
3:1 (insert:vector) ratio
D186 (insert) 0 µL 1 µL
D187 (vector) 2,5 µL 2,5 µL
T4 DNA ligase 10X buffer 2 µL 2 µL
T4 DNA ligase 1 µL 1 µL
water 14,5 µL 13,5 µL
  • Put in the room "Danger azote" at about 16°C at 18h00 (for overnight reaction).
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