Team:Paris/September 4

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(Difference between revisions)
(Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3))
(Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3))
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Revision as of 15:44, 4 September 2008

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Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3)

Ligation L173

  • insert: D112 (ES) rbs-TetR
  • vector: D187 (EX) mRFP-LVA-tripart-pSB1A3
Sample positive control
pUC19
negative control
no DNA
ligation control
(without insert)
L173 (3:1 ratio) L173 (4:1 ratio)
Number of colonies many 1 0 2 0


L177 = d134 + d187 (pFlhB-mRFP LVA+)

L175 = d149 + d° (pFliL - ECFP LVA+)

L176 = d149 + d° (pFliL - ECFP LVA-)